Analytical Data
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Gene name
HEXA/Hexosaminidase A
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简介
HEXA (hexosaminidase A) proteolytically hydrolyzes N-acetyl-D-hexosamine and/or sulfated residues in glycocomplexes, including oligosaccharides, glycolipids, and mucopolysaccharides. The activity of isoenzyme S towards anionic disulfated glycans is comparable to that of isoenzyme A, while isoenzyme B can effectively hydrolyze neutral oligosaccharides but cannot hydrolyze anionic disulfated substrates. HEXA/Hexosaminidase A Protein, Human (sf9, His) is the recombinant human-derived HEXA/Hexosaminidase A protein, expressed by Sf9 insect cells , with C-His labeled tag.
- Application
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Alternative Names
Beta-hexosaminidase subunit alpha; HEXA; Hexosaminidase A; TSD
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Species
Human
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Source
Baculovirus
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Tag
C-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
AAD13932.1
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Expression Region
L23-T529
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AA Sequence
MTSSRLWFSLLLAAAFAGRATALWPWPQNFQTSDQRYVLYPNNFQFQYDVSSAAQPGCSVLDEAFQRYRDLLFGSGSWPRPYLTGKRHTLEKNVLVVSVVTPGCNQLPTLESVENYTLTINDDQCLLLSETVWGALRGLETFSQLVWKSAEGTFFINKTEIEDFPRFPHRGLLLDTSRHYLPLSSILDTLDVMAYNKLNVFHWHLVDDPSFPYESFTFPELMRKGSYNPVTHIYTAQDVKEVIEYARLRGIRVLAEFDTPGHTLSWGPGIPGLLTPCYSGSEPSGTFGPVNPSLNNTYEFMSTFFLEVSSVFPDFYLHLGGDEVDFTCWKSNPEIQDFMRKKGFGEDFKQLESFYIQTLLDIVSSYGKGYVVWQEVFDNKVKIQPDTIIQVWREDIPVNYMKELELVTKAGFRALLSAPWYLNRISYGPDWKDFYIVEPLAFEGTPEQKALVIGGEACMWGEYVDNTNLVPRLWPRAGAVAERLWSNKLTSDLTFAYERLSHFRCELLRRGVQAQPLNVGFCEQEFEQT
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Protein Length
Partial
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Molecular Weight
64 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Hexosaminidase A (HEXA) is an essential enzyme involved in the metabolism of glycosphingolipids, primarily in the brain and other tissues. Its deficiency leads to Tay-Sachs disease, a severe genetic disorder characterized by neurodegeneration and early childhood mortality. The HEXA gene encodes the alpha subunit of the Hexosaminidase A enzyme, and mutations in this gene result in partial or complete loss of enzymatic activity. Research into recombinant HEXA protein has gained traction as a potential therapeutic approach for Tay-Sachs and related disorders. By producing HEXA in a laboratory setting, scientists aim to develop enzyme replacement therapies that could supplement the missing or malfunctioning enzyme in affected individuals. Advances in biotechnological methods such as recombinant DNA technology have made it possible to express and purify functional HEXA for use in preclinical and clinical studies. Recent efforts focus on optimizing the production process, improving enzyme stability and delivery, and understanding the biochemical properties of the recombinant protein to ensure it can effectively cross the blood-brain barrier. As the global scientific community continues to investigate gene therapy and enzyme replacement options, HEXA research remains at the forefront of advancements aimed at alleviating the burden of lysosomal storage disorders and improving patient outcomes.
