Analytical Data
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基因名
FSTL3
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简介
In its secreted form, the FSTL3 protein acts as a binder and antagonist to TGF-β family members, inhibiting cell signaling induced by activin A, activin B, BMP2, and MSTN. It plays a role in bone formation, inhibition of osteoclast differentiation, hematopoiesis, and enhanced hematopoietic progenitor cell adhesion. FSTL3 Protein, Mouse (HEK293, His) is the recombinant mouse-derived FSTL3 protein, expressed by HEK293 , with C-His labeled tag.
- Application
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生物活性
1.Immobilized Human Activin A, No Tag at 0.2 μg/mL (100 μl/well) on the plate. Dose response curve for Mouse FSTL3, His Tag with the EC50 of >20.1 ng/mL determined by ELISA. 2.Measured by its binding ability in a functional ELISA. Immobilized mouse FLRG-His at 10 μg/mL (100 μL/well) can bind biotinylated mouse INHBA, the ED50 for this effect is 21.37 ng/mL. Measured by its binding ability in a functional ELISA. Immobilized mouse FLRG-His at 10 μg/mL (100 μL/well) can bind biotinylated mouse INHBA,The ED50 for this effect is 21.37 ng/mL.
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别名
Follistatin-related protein 3; Follistatin-like protein 3; FLRG
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种属
Mouse
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表达系统
HEK293
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标签
C-His
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
Q9EQC7
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表达区间
V24-V256
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氨基酸序列
VMGSEDSVPGGVCWLQQGREATCSLVLKTRVSREECCASGNINTAWSNFTHPGNKISLLGFLGLVHCLPCKDSCDGVECGPGKACRMLGGRPHCECVPNCEGLPAGFQVCGSDGATYRDECELRTARCRGHPDLRVMYRGRCQKSCAQVVCPRPQSCLVDQTGSAHCVVCRAAPCPVPSNPGQELCGNNNVTYISSCHLRQATCFLGRSIGVRHPGICTGGPKVPAEEEENFV
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蛋白长度
Full Length of Mature Protein
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分子量
35-40 kDa.
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
FSTL3 (Follistatin-like 3) is a secreted glycoprotein belonging to the follistatin family, known for its role in modulating various biological processes, including inflammation, tissue repair, and metabolic regulation. Increased interest in FSTL3 has emerged due to its involvement in several pathophysiological conditions, such as obesity, diabetes, and cardiovascular diseases. Research indicates that FSTL3 may play a protective role against the progression of these diseases by influencing insulin sensitivity and inflammatory responses. Consequently, the recombinant production of FSTL3 protein has gained momentum, allowing for in-depth studies of its biological functions and potential therapeutic applications. Recombinant forms facilitate the investigation of FSTL3's mechanism of action, as well as its role in cellular signaling pathways and interactions with other proteins. The ability to produce FSTL3 in sufficient quantities also paves the way for drug development and biomarker discovery, with implications for improving patient outcomes in metabolic and inflammatory diseases. Overall, the exploration of FSTL3 as a recombinant protein is essential for understanding its roles in health and disease, and it holds promise for innovations in clinical treatments.
