Analytical Data
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Gene name
Neurolysin
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简介
Neurolysin protein is a multifunctional enzyme that hydrolyzes oligopeptides such as neurotensin, bradykinin, and dynorphin A. In addition to its role in oligopeptide metabolism, Neurolysin also modulates cannabinoid signaling pathways by promoting the degradation of vasopressin, an antagonist peptide of the cannabinoid receptor CNR1. Neurolysin Protein, Human (His) is the recombinant human-derived Neurolysin protein, expressed by E. coli , with N-His labeled tag.
- Application
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Alternative Names
Neurolysin, mitochondrial; Microsomal endopeptidase; MEP; NLN; AGTBP; KIAA1226
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9BYT8
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Expression Region
S38-P704
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AA Sequence
SSYTVAGRNVLRWDLSPEQIKTRTEELIVQTKQVYDAVGMLGIEEVTYENCLQALADVEVKYIVERTMLDFPQHVSSDKEVRAASTEADKRLSRFDIEMSMRGDIFERIVHLQETCDLGKIKPEARRYLEKSIKMGKRNGLHLPEQVQNEIKSMKKRMSELCIDFNKNLNEDDTFLVFSKAELGALPDDFIDSLEKTDDDKYKITLKYPHYFPVMKKCCIPETRRRMEMAFNTRCKEENTIILQQLLPLRTKVAKLLGYSTHADFVLEMNTAKSTSRVTAFLDDLSQKLKPLGEAEREFILNLKKKECKDRGFEYDGKINAWDLYYYMTQTEELKYSIDQEFLKEYFPIEVVTEGLLNTYQELLGLSFEQMTDAHVWNKSVTLYTVKDKATGEVLGQFYLDLYPREGKYNHAACFGLQPGCLLPDGSRMMAVAALVVNFSQPVAGRPSLLRHDEVRTYFHEFGHVMHQICAQTDFARFSGTNVETDFVEVPSQMLENWVWDVDSLRRLSKHYKDGSPIADDLLEKLVASRLVNTGLLTLRQIVLSKVDQSLHTNTSLDAASEYAKYCSEILGVAATPGTNMPATFGHLAGGYDGQYYGYLWSEVFSMDMFYSCFKKEGIMNPEVGMKYRNLILKPGGSLDGMDMLHNFLKREPNQKAFLMSRGLHAP
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Protein Length
Full Length of Mature Protein
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Molecular Weight
67 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Neurolysin is a metalloprotease enzyme predominantly expressed in the nervous system, playing a critical role in the degradation of neuropeptides and several bioactive peptides. Its function is essential for maintaining neuronal homeostasis and modulating various neurophysiological processes, including neuroinflammation and neuroprotection. Recent studies have revealed that neurolysin is involved in neurodegenerative diseases, such as Alzheimer's and Parkinson's, where dysregulation of peptide metabolism may contribute to disease pathology. The ability of neurolysin to selectively degrade harmful peptides opens avenues for therapeutic interventions aimed at enhancing its activity or mimicking its function. This has led to an increasing interest in recombinant neurolysin, which is produced by genetic engineering to facilitate detailed studies of its structure, function, and potential therapeutic implications. Understanding the biochemical properties and regulatory mechanisms of neurolysin can provide insights into its role in the central nervous system and highlight the potential for developing neuroprotective strategies targeting this enzyme in disease contexts.
