Recombinant Mouse Activin A Protein(HEK293), C-6*His

Activin A

Activin A, a multifunctional cytokine, is a member of TGF-β superfamily. Activin A first binds to the type II activin receptors (ActIIRA or ActRIIB) on the member surface, and then recruits and phosphorylates type I activin receptors (ActRI). Activin A primarily signal through SMAD2/3 proteins to regulate a variety of functions, including inflammation, fibrosis, and tumorigenesis. Activin A Protein, Mouse (HEK293, His) is produced in HEK293 cells with a C-Terminal His-tag.

SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

1.Measured in a cell proliferation assay using HT-29 human colon cancer cell. The ED50 for this effect is 6.407 ng/mL, corresponding to a specific activity is 1.561×105 units/mg. 2.Measured by its ability to inhibit proliferation of MPC-11 cells. The ED50 for this effect is typically ≤30 μg/mL. 3.Measured by its ability in a ELISA. 3.1. Immobilized human ACVR2B at 10 μg/mL (100 μl/well) can bind biotinylated mouse INHBA-His, The EC50 of biotinylated mouse INHBA-His is ≤0.2 μg/mL. 3.2 Immobilized mouse INHBA-his at 10 μg/mL (100 μl/well) can bind human Follistatin Protein, The EC50 of human Follistatin Protein is ≤0.4 μg/mL. Measured in a cell proliferation assay using HT-29 human colon cancer cell. The ED50 for this effect is 6.407 ng/mL, corresponding to a specific activity is 1.561×105 units/mg.

Activin beta-A chain; Activin A; Inhibin beta A chain; INHBA

Mouse

HEK293

C-6*His

Greater than 95% as determined by reducing SDS-PAGE.

Q3UY39

S21-S424

Full Length of Mature Protein

18 kDa & 43 kDa

< 1.0 EU per μg protein as determined by the LAL method.

Freeze-dried powder

PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.