Analytical Data
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基因名
Activin A
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简介
Activin A, a multifunctional cytokine, is a member of TGF-β superfamily. Activin A first binds to the type II activin receptors (ActIIRA or ActRIIB) on the member surface, and then recruits and phosphorylates type I activin receptors (ActRI). Activin A primarily signal through SMAD2/3 proteins to regulate a variety of functions, including inflammation, fibrosis, and tumorigenesis. Activin A Protein, Mouse (HEK293, His) is produced in HEK293 cells with a C-Terminal His-tag.
- Application
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生物活性
1.Measured in a cell proliferation assay using HT-29 human colon cancer cell. The ED50 for this effect is 6.407 ng/mL, corresponding to a specific activity is 1.561×105 units/mg. 2.Measured by its ability to inhibit proliferation of MPC-11 cells. The ED50 for this effect is typically ≤30 μg/mL. 3.Measured by its ability in a ELISA. 3.1. Immobilized human ACVR2B at 10 μg/mL (100 μl/well) can bind biotinylated mouse INHBA-His, The EC50 of biotinylated mouse INHBA-His is ≤0.2 μg/mL. 3.2 Immobilized mouse INHBA-his at 10 μg/mL (100 μl/well) can bind human Follistatin Protein, The EC50 of human Follistatin Protein is ≤0.4 μg/mL. Measured in a cell proliferation assay using HT-29 human colon cancer cell. The ED50 for this effect is 6.407 ng/mL, corresponding to a specific activity is 1.561×105 units/mg.
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别名
Activin beta-A chain; Activin A; Inhibin beta A chain; INHBA
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种属
Mouse
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表达系统
HEK293
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标签
C-6*His
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纯度
Greater than 95% as determined by reducing SDS-PAGE.
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蛋白编号
Q3UY39
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表达区间
S21-S424
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蛋白长度
Full Length of Mature Protein
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分子量
18 kDa & 43 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Activin A, a member of the transforming growth factor-beta (TGF-β) superfamily, plays a crucial role in various biological processes, including embryonic development, cell differentiation, and tissue repair. Its importance has garnered significant attention in both fundamental and clinical research. Originally identified for its regulatory functions in reproductive biology, Activin A has also been implicated in inflammation, metabolism, and the immune response, highlighting its potential as a therapeutic target. Recombinant Activin A protein has been developed for various applications, facilitating studies on its signaling pathways and physiological roles. Researchers are exploring its effects in diseases such as cancer, fibrosis, and autoimmune disorders. The ability to produce Activin A in a recombinant form allows for more precise studies of its functions and interactions, paving the way for novel treatment strategies. Furthermore, understanding its roles in stem cell pluripotency and differentiation can enhance regenerative medicine approaches. The ongoing investigations into Activin A highlight its promise as a multifaceted mediator in health and disease, emphasizing the need for continued research to fully elucidate its mechanisms of action and therapeutic potential.
