Analytical Data
-
Gene name
Tubulin Beta
- Application
-
Alternative Names
TUBB5; TUBB
-
Species
Human
-
Source
E. coli
-
Tag
GST
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
P07437
-
Expression Region
M1-A444
-
Protein Length
Full Length
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Tubulin beta is a crucial component of the cytoskeleton in eukaryotic cells, playing a pivotal role in microtubule formation and stability, which are essential for various cellular functions, including mitosis, intracellular transport, and cell shape maintenance. Research on recombinant tubulin beta proteins has gained significant traction due to their potential applications in understanding cytoskeletal dynamics and drug development. Microtubules, composed of alpha and beta tubulin heterodimers, are dynamic structures that undergo rapid polymerization and depolymerization, a process regulated by various cellular factors. The recombinant expression of tubulin beta allows for in-depth studies into its biophysical properties, interactions with microtubule-associated proteins, and response to anti-cancer drugs targeting microtubule stability, such as taxanes and vinca alkaloids. Moreover, the ability to manipulate and analyze these proteins in vitro facilitates the elucidation of the molecular mechanisms underpinning cell division and the implications of tubulin mutations linked to diseases. As such, the study of recombinant tubulin beta protein not only enhances our understanding of fundamental biological processes but also aids in the development of therapeutic strategies for cancer and other diseases characterized by dysregulated cell proliferation.
