Analytical Data
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Gene name
Profilin-2
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简介
Profilin-2 protein is a key player in protein folding. It specifically binds to cytosolic chaperone protein (c-CPN) and promotes the transfer of target proteins. It also interacts with nascent polypeptide chains, helping unnatural proteins fold correctly in various competing pathways. Profilin-2 Protein, Human (His) is the recombinant human-derived Profilin-2 protein, expressed by E. coli , with N-6*His labeled tag.
- Application
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Alternative Names
Profilin-II; PFN2; Profilin-2; PFL
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Species
Human
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Source
E. coli
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Tag
N-6*His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
AAH18049.1
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Expression Region
M1-F140
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Protein Length
Full Length
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Molecular Weight
16 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Profilin-2 is an essential actin-binding protein that plays a crucial role in cytoskeletal dynamics and cellular processes such as cell motility, division, and signal transduction. It is part of the profilin family, which is known for its ability to regulate actin polymerization by binding to G-actin and promoting the formation of F-actin. Research on Profilin-2 has gained prominence due to its implications in various physiological and pathological conditions, including cancer, neurodegenerative diseases, and cardiac dysfunction. Variations in Profilin-2 expression and function can significantly affect cellular architecture and mobility, making it a potential biomarker for disease progression. In the context of recombinant protein studies, Profilin-2 has been extensively investigated for its biochemical properties, interaction with other proteins, and effects on actin dynamics. The production of recombinant Profilin-2 allows for a deeper understanding of its structural characteristics and functional mechanisms, providing insights that could lead to novel therapeutic strategies aimed at modulating cytoskeletal functions in disease contexts. Through mutagenesis and other biochemical assays, researchers are exploring the specific domains of Profilin-2 that mediate its interactions with actin and other signaling pathways. This body of work not only contributes to a comprehensive understanding of Profilin-2's role in cellular biology but also paves the way for potential applications in drug development and targeted therapies for diseases linked to cytoskeletal dysfunction.
