Analytical Data
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Gene name
MRE11A
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简介
The MRE11A protein is an important component of the DNA double-strand break repair mechanism and participates in the homologous recombination (HR) pathway. It interacts with RAD50 and NBS1 to form the MRN complex, which is essential for sensing and repairing DNA damage. MRE11A Protein, Human (His, GST) is the recombinant human-derived MRE11A protein, expressed by E. coli , with N-6*His, N-GST labeled tag.
- Application
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Alternative Names
MRE11; Double-strand break repair protein MRE11; Double-strand break repair protein MRE11A; Meiotic recombination 11 homolog 1; MRE11 homolog 1; Meiotic recombination 11 homolog A; MRE11 homolog A
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Species
Human
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Source
E. coli
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Tag
N-6*His;N-GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P49959
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Expression Region
M1-E411
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Protein Length
Partial
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Molecular Weight
74.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
MRE11A, a critical component of the MRN (MRE11-RAD50-NBS1) complex, plays a vital role in the cellular response to DNA damage, particularly in the repair of double-strand breaks (DSBs). This protein possesses both exonuclease and endonuclease activities, allowing it to process DNA ends during repair processes such as homologous recombination and non-homologous end joining. Research on MRE11A has gained significant attention due to its implications in genomic stability, cancer biology, and various genetic disorders. Mutations or dysregulation of MRE11A are linked to increased susceptibility to cancers, including breast and prostate cancers, along with neurodegenerative diseases. Moreover, its involvement in the DNA damage response pathways makes it a promising target for therapeutic strategies aimed at enhancing the efficacy of radiotherapy and chemotherapy. Studies have also shown that MRE11A is crucial for telomere maintenance, further highlighting its importance in cellular longevity and aging processes. Understanding the functional dynamics of MRE11A at the molecular level could yield insights not only into its role in disease pathogenesis but also into potential biomarkers for cancer prognosis and treatment outcomes. Recent advances in structural biology and biochemistry have facilitated in-depth investigations into the interactions of MRE11A with other proteins and DNA substrates, paving the way for novel research into its regulatory mechanisms and potential applications in gene therapy and precision medicine. This multifaceted role of MRE11A underscores its significance as a focal point for research into DNA repair and cancer therapies, making it an important subject of ongoing scientific inquiry.
