Analytical Data
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基因名
PPA1
- Application
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别名
(Pyrophosphate phospho-hydrolase)(PPase)
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种属
Human
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表达系统
E. coli
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标签
N- His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q15181
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表达区间
228-289aa
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分子量
10.8 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PPA1, or phosphatase PPA1, is an essential protein that plays a crucial role in cellular phosphate metabolism, particularly in the context of mitochondrial function and energy production. Research into PPA1 has gained traction due to its involvement in various physiological processes and diseases, including potential links to metabolic disorders and mitochondrial dysfunction. The enzyme is responsible for dephosphorylating inorganic pyrophosphate (PPi), thereby influencing ATP synthesis and overall cellular energy balance. Dysfunction in PPA1 has been associated with compromised mitochondrial efficiency, which can lead to various health complications. Recent studies have focused on the recombinant expression of PPA1 in heterologous systems, allowing for detailed characterization of its biochemical properties and regulation. By generating recombinant PPA1, researchers seek to elucidate its structure-function relationships and interaction with other metabolic pathways. Furthermore, the availability of purified PPA1 opens avenues for the investigation of therapeutic targets in diseases where energy metabolism is disrupted. Understanding the role of PPA1 in cellular processes not only enhances our knowledge of basic biology but also has potential implications for designing interventions in metabolic diseases, thus emphasizing the importance of this protein in both health and disease contexts. Overall, the study of PPA1 and its recombinant forms represents a significant step forward in biochemistry and molecular biology, with the potential to provide insights that may translate into clinical relevance.












