Analytical Data
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Gene name
SNAP-alpha
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简介
SNAP-α proteins are critical in vesicle trafficking, mediating dynamic exchange between the endoplasmic reticulum and the Golgi apparatus. It cooperates with GNA12 to localize CDH5 to the plasma membrane. SNAP-alpha Protein, Human (His) is the recombinant human-derived SNAP-alpha protein, expressed by E. coli , with N-6*His labeled tag.
- Application
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Alternative Names
Alpha-Soluble NSF Attachment Protein; SNAP-Alpha; N-Ethylmaleimide-Sensitive Factor Attachment Protein Alpha; NAPA; SNAPA
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Species
Human
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Source
E. coli
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Tag
N-6*His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
P54920
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Expression Region
M1-R295
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AA Sequence
MDNSGKEAEAMALLAEAERKVKNSQSFFSGLFGGSSKIEEACEIYARAANMFKMAKNWSAAGNAFCQAAQLHLQLQSKHDAATCFVDAGNAFKKADPQEAINCLMRAIEIYTDMGRFTIAAKHHISIAEIYETELVDIEKAIAHYEQSADYYKGEESNSSANKCLLKVAGYAALLEQYQKAIDIYEQVGTNAMDSPLLKYSAKDYFFKAALCHFCIDMLNAKLAVQKYEELFPAFSDSRECKLMKKLLEAHEEQNVDSYTESVKEYDSISRLDQWLTTMLLRIKKTIQGDEEDLR
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Protein Length
Full Length
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Molecular Weight
33-35 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SNAP-alpha, a member of the SNAP (SNAp25-like protein) family, plays a crucial role in the regulation of neurotransmitter release at synapses. It is particularly involved in the process of exocytosis, where vesicles containing neurotransmitters fuse with the plasma membrane to release their contents into the synaptic cleft. Research has shown that SNAP-alpha works alongside other proteins, such as syntaxins and VAMPs, to facilitate the precise orchestration of this process, making it essential for effective neuronal communication. Understanding SNAP-alpha's structure and function is critical, as dysregulation of its activity can lead to various neurological disorders, including schizophrenia, depression, and neurodegenerative diseases. The study of SNAP-alpha and its interactions also provides insights into fundamental cellular mechanisms and potential therapeutic targets for drug development. Recent advancements in recombinant protein expression techniques have enabled researchers to produce and study SNAP-alpha in detail, investigating its role in synaptic function, protein-protein interactions, and post-translational modifications. These studies are vital for elucidating the molecular underpinnings of neurotransmission and may pave the way for innovative strategies to modulate synaptic activity in pathological conditions.
