Analytical Data
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基因名
CRKL
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简介
CRKL protein mediates intracellular signal transduction, interacts with tyrosine-phosphorylated EPOR and INPP5D/SHIP1, and plays a role in erythropoietin receptor and phosphoinositide signaling. It also binds to DOCK2 and DOCK5, suggesting regulation of guanine nucleotide exchange factors. CRKL Protein, Human (His, Strep) is the recombinant human-derived CRKL protein, expressed by E. coli , with N-Strep, N-6*His labeled tag.
- Application
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别名
CRKL; Crk-like protein
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种属
Human
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表达系统
E. coli
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标签
N-StrepⅡ;N-6*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P46109
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表达区间
M1-E303
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蛋白长度
Full Length
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CRKL (Crk-like protein) is a pivotal adaptor protein implicated in various cellular processes, including signal transduction, cytoskeletal organization, and cell migration. It's a member of the Crk family of proteins, which are characterized by SH2 (Src Homology 2) and SH3 (Src Homology 3) domains that facilitate interaction with diverse signaling molecules. The study of CRKL has gained significance due to its role in cancer biology, particularly in connection with the phosphorylation-dependent signaling pathways influenced by receptor tyrosine kinases and oncogenic proteins. Dysregulation of CRKL has been linked to several malignancies, suggesting that targeting CRKL may offer therapeutic potential. Recent advancements in recombinant protein technology have enabled the production of purified CRKL for biochemical and structural studies, providing insights into its functional mechanisms and interactions in cellular contexts. Understanding the structural dynamics and functional roles of CRKL can elucidate its contribution to oncogenic signaling, advance the development of CRKL-targeted therapies, and improve therapeutic strategies in cancer treatment. Hence, ongoing research focuses on the detailed characterization of CRKL, aiming to uncover novel regulatory pathways and interactions that may be harnessed for clinical applications in oncology.
