Analytical Data
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Gene name
P4HB
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简介
The P4HB protein is a multifunctional entity that catalyzes disulfide bonding processes at the cell surface and within cells. As a reductase, it modifies outer surface proteins and participates in the formation of disulfide bonds in nascent proteins. P4HB Protein, Human (HEK293, His) is the recombinant human-derived P4HB protein, expressed by HEK293 , with C-6*His labeled tag.
- Application
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Biological Activity
Measured by its ability to promote aggregation of insulin in the presence of DTT. The specific activity is >7.5 A650/cm/min/mg, as measured underthe described conditions.
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Alternative Names
Protein Disulfide-Isomerase; PDI; Cellular Thyroid Hormone-Binding Protein; Prolyl 4-Hydroxylase Subunit Beta; p55; P4HB; ERBA2L; PDI; PDIA1; PO4DB
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Species
Human
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Source
HEK293
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Tag
C- 10*His tag
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P07237
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Expression Region
18-505aa
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Molecular Weight
60 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Identification
Protein Description
P4HB, also known as prolyl 4-hydroxylase beta subunit, is a pivotal protein involved in collagen synthesis and folding, playing a crucial role in the formation of the extracellular matrix (ECM). The study of P4HB has garnered significant attention due to its essential function in various physiological processes, including tissue repair, wound healing, and the regulation of hypoxia responses. Dysregulation of P4HB is implicated in several pathological conditions, such as fibrosis, cancer, and cardiovascular diseases. Research has focused on elucidating its mechanistic role in these processes, particularly its interaction with other molecular chaperones and enzymes. Furthermore, P4HB's potential as a therapeutic target has been explored, with studies investigating how modulating its activity could lead to innovative treatments for related diseases. Understanding the structure and function of P4HB is therefore critical, paving the way for advancements in biomedical applications and improving therapeutic strategies aimed at mitigating the effects of ECM-related disorders.
