Analytical Data
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Gene name
G-CSFR/CD114
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简介
The G-CSFR/CD114 protein, as a receptor for granulocyte colony-stimulating factor (CSF3), is essential for granulocyte maturation and plays a key role in the proliferation, differentiation, and survival of neutrophil lineage cells. Furthermore, it may be involved in cell surface adhesion or recognition events. G-CSFR/CD114 Protein, Human (HEK293) is the recombinant human-derived G-CSFR/CD114 protein, expressed by HEK293 , with tag free.
- Application
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Biological Activity
Measured by its ability to inhibit the G-CSF-induced proliferation of M-NFS-60 cells. The ED50 for this effect is 1.299 μg/mL in the presence of 0.125 ng/mL of recombinant human G-CSF, corresponding to a specific activity is 769.82 units/mg. Measured by its ability to inhibit the G-CSF-induced proliferation of M-NFS‑60 cells. The ED50 for this effect is 1.299 μg/mL in the presence of 0.125 ng/mL of recombinant human G-CSF, corresponding to a specific activity is 769.82 units/mg.
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Alternative Names
Granulocyte colony-stimulating factor receptor; G-CSF-R; CD114; CSF3R; GCSFR
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Species
Human
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Source
HEK293
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Tag
Tag Free
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
Q99062
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Expression Region
E25-P621
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AA Sequence
ECGHISVSAPIVHLGDPITASCIIKQNCSHLDPEPQILWRLGAELQPGGRQQRLSDGTQESIITLPHLNHTQAFLSCCLNWGNSLQILDQVELRAGYPPAIPHNLSCLMNLTTSSLICQWEPGPETHLPTSFTLKSFKSRGNCQTQGDSILDCVPKDGQSHCCIPRKHLLLYQNMGIWVQAENALGTSMSPQLCLDPMDVVKLEPPMLRTMDPSPEAAPPQAGCLQLCWEPWQPGLHINQKCELRHKPQRGEASWALVGPLPLEALQYELCGLLPATAYTLQIRCIRWPLPGHWSDWSPSLELRTTERAPTVRLDTWWRQRQLDPRTVQLFWKPVPLEEDSGRIQGYVVSWRPSGQAGAILPLCNTTELSCTFHLPSEAQEVALVAYNSAGTSRPTPVVFSESRGPALTRLHAMARDPHSLWVGWEPPNPWPQGYVIEWGLGPPSASNSNKTWRMEQNGRATGFLLKENIRPFQLYEIIVTPLYQDTMGPSQHVYAYSQEMAPSHAPELHLKHIGKTWAQLEWVPEPPELGKSPLTHYTIFWTNAQNQSFSAILNASSRGFVLHGLEPASLYHIHLMAASQAGATNSTVLTLMTLTP
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Protein Length
Partial
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Molecular Weight
85.36 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
G-CSFR (Granulocyte Colony-Stimulating Factor Receptor) or CD114 is a crucial protein that plays a significant role in hematopoiesis, particularly in the regulation of granulocyte production and differentiation from progenitor cells. The interaction between G-CSF and its receptor facilitates the proliferation and survival of neutrophils, which are vital components of the immune response. Dysregulation of G-CSFR signaling has been implicated in various hematological disorders, such as neutropenia and leukemia, highlighting the importance of understanding its molecular structure and function. Recent advancements in biotechnology have enabled the production of recombinant G-CSFR/CD114 proteins, providing valuable tools for both basic research and therapeutic applications. These recombinant proteins can be utilized in functional assays to decipher the signaling pathways involved in myeloid cell development, assess the receptor's role in disease pathogenesis, and investigate potential targets for pharmacological intervention. Furthermore, G-CSFR/CD114 recombinant proteins hold promise in enhancing the effectiveness of G-CSF in clinical settings, particularly for patients undergoing chemotherapy or bone marrow transplantation. Continued research on G-CSFR/CD114 and its recombinant forms is expected to yield significant insights into immune regulation and improve therapeutic strategies for related disorders.
