Analytical Data
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基因名
SLC14A1
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简介
SLC14A1 Protein plays a pivotal role in cellular processes, mediating urea transport across erythrocyte and renal inner medullary collecting duct membranes. It is crucial for the urinary concentrating mechanism and regulates water transport in erythrocytes. With a dual role in urea transport, SLC14A1 maintains cellular osmotic balance and contributes to physiological processes associated with urinary concentration, highlighting its significance. SLC14A1 Protein, Human (sf9, His, MBP, FLAG) is the recombinant human-derived SLC14A1 protein, expressed by sf9 insect cells , with N-MBP, C-Flag, N-8*His labeled tag.
- Application
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别名
SLC14A1; Urea transporter 1; Solute carrier family 14 member 1; Urea transporter; erythrocyte
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种属
Human
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表达系统
Baculovirus
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标签
N-MBP;C-Flag;N-8*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q13336
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表达区间
E2-L389
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蛋白长度
Partial
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SLC14A1, also known as the solute carrier family 14 member 1, is a crucial protein involved in the transport of urea across cellular membranes, playing a significant role in nitrogen metabolism and homeostasis. Mutations in the SLC14A1 gene have been linked to various genetic disorders, notably hereditary hypoammonemia and specific renal pathologies, which highlight its importance in both physiological and pathological contexts. Research into the recombinant SLC14A1 protein has gained momentum due to its potential applications in understanding the mechanisms of urea transport and the development of therapeutic interventions for associated diseases. By producing and characterizing recombinant SLC14A1, scientists aim to delineate its structural and functional properties, uncover its transport kinetics, and explore its interactions with other cellular components. This knowledge could pave the way for novel strategies to manage conditions arising from SLC14A1 dysfunction. Additionally, investigating this protein could contribute to the broader field of membrane transport systems, offering insights into how cells regulate the movement of key metabolites and maintain homeostasis in various physiological states. Ongoing studies focus on optimizing recombinant protein production methods, improving purification processes, and utilizing advanced techniques such as crystallography and cryo-electron microscopy to elucidate the protein's 3D structure, which is vital for understanding its function and facilitating the design of targeted therapies. Overall, the research on SLC14A1 recombinant protein is not only significant for elucidating fundamental physiological mechanisms but also holds promise for advancing medical interventions in related disorders.
