Analytical Data
-
Gene name
APC
-
简介
The APC protein is a multifaceted tumor suppressor that rapidly degrades CTNNB1 and acts as a negative regulator in the Wnt pathway. Its phosphorylation status intricately affects activity. APC Protein, Human (His) is the recombinant human-derived APC protein, expressed by E. coli , with N-6*His labeled tag.
- Application
-
Alternative Names
APC; Adenomatous polyposis coli protein; Protein APC; Deleted in polyposis 2.5
-
Species
Human
-
Source
E. coli
-
Tag
N-6*His
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
P25054
-
Expression Region
L407-S751
-
Protein Length
Partial
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
APC (Adenomatous Polyposis Coli) is a crucial tumor suppressor protein that plays a vital role in regulating cell adhesion, migration, and proliferation, as well as maintaining genomic stability. Mutations in the APC gene are primarily associated with familial adenomatous polyposis (FAP), a hereditary condition that significantly increases the risk of colorectal cancer. Research into APC has intensified due to its profound implications in cancer biology; investigating its pathways reveals insights into tumorigenesis. APC functions as a key regulator of the Wnt signaling pathway, which, when aberrantly activated, leads to uncontrolled cell growth and cancer development. APC protein interactions with various partners contribute to its role in β-catenin degradation, and disruptions in these interactions can result in the accumulation of β-catenin and subsequent tumorigenesis. Furthermore, APC's involvement in the cellular cytoskeleton and mitotic spindle dynamics emphasizes its multifaceted roles in maintaining cellular integrity. Exploring the structural and functional dynamics of APC through recombinant protein studies allows for a deeper understanding of its mechanisms and potential as a therapeutic target. Continuous advancements in protein engineering techniques facilitate the production of functional APC variants, supporting investigations into its interactions and regulatory roles, thus contributing to enhanced strategies for cancer prevention and treatment.
