Analytical Data
-
基因名
APC
-
简介
The APC protein is a multifaceted tumor suppressor that rapidly degrades CTNNB1 and acts as a negative regulator in the Wnt pathway. Its phosphorylation status intricately affects activity. APC Protein, Human (His) is the recombinant human-derived APC protein, expressed by E. coli , with N-6*His labeled tag.
- Application
-
别名
APC; Adenomatous polyposis coli protein; Protein APC; Deleted in polyposis 2.5
-
种属
Human
-
表达系统
E. coli
-
标签
N-6*His
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
P25054
-
表达区间
L407-S751
-
蛋白长度
Partial
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
APC (Adenomatous Polyposis Coli) is a crucial tumor suppressor protein that plays a vital role in regulating cell adhesion, migration, and proliferation, as well as maintaining genomic stability. Mutations in the APC gene are primarily associated with familial adenomatous polyposis (FAP), a hereditary condition that significantly increases the risk of colorectal cancer. Research into APC has intensified due to its profound implications in cancer biology; investigating its pathways reveals insights into tumorigenesis. APC functions as a key regulator of the Wnt signaling pathway, which, when aberrantly activated, leads to uncontrolled cell growth and cancer development. APC protein interactions with various partners contribute to its role in β-catenin degradation, and disruptions in these interactions can result in the accumulation of β-catenin and subsequent tumorigenesis. Furthermore, APC's involvement in the cellular cytoskeleton and mitotic spindle dynamics emphasizes its multifaceted roles in maintaining cellular integrity. Exploring the structural and functional dynamics of APC through recombinant protein studies allows for a deeper understanding of its mechanisms and potential as a therapeutic target. Continuous advancements in protein engineering techniques facilitate the production of functional APC variants, supporting investigations into its interactions and regulatory roles, thus contributing to enhanced strategies for cancer prevention and treatment.
