Analytical Data
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Gene name
VKOR1
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简介
As the catalytic subunit of the VKOR complex, the VKOR1 protein is crucial in vitamin K metabolism and is essential for the reduction of inactive vitamin K 2,3-epoxide to the active form. This process is critical for γ-carboxylation of proteins and is particularly important for blood coagulation and the synthesis of coagulation factors. VKOR1 Protein, Human (HEK293, GFP, His) is the recombinant human-derived VKOR1 protein, expressed by HEK293 , with C-10*His, GFP labeled tag.
- Application
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Alternative Names
VKORC1; Vitamin K epoxide reductase complex subunit 1; Vitamin K1 2; 3-epoxide reductase subunit 1
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Species
Human
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Source
HEK293
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Tag
C-10*His;GFP
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9BQB6
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Expression Region
M1-H163
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Protein Length
Full Length
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
VKOR1 (Vitamin K Epoxide Reductase 1) plays a crucial role in the vitamin K cycle, facilitating the regeneration of reduced vitamin K, which is essential for the post-translational modification of several proteins involved in blood coagulation. The enzyme's dysfunction can lead to various coagulation disorders and has been implicated in resistance to anticoagulant medications such as warfarin. As a result, understanding VKOR1 at the molecular level is vital for advancing therapeutic strategies in managing coagulation-related diseases. The recombinant expression of VKOR1 in various systems, such as bacteria, yeast, or mammalian cells, allows researchers to obtain the protein in sufficient quantities for biochemical and structural studies. These studies aim to elucidate the enzyme's mechanism of action, identify key residues involved in its function, and assess its interaction with vitamin K and anticoagulants. Furthermore, recombinant VKOR1 serves as a valuable tool for drug screening and the design of novel anticoagulants with improved specificity and reduced side effects. Overall, the research on VKOR1 recombinant proteins holds significant promise for enhancing our understanding of coagulation processes and developing better therapeutic options for patients with coagulation disorders.
