Analytical Data
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基因名
hERG
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简介
The hERG protein is the α subunit of voltage-gated potassium channels and is critical for mediating cardiac delayed rectifier potassium current (IKr). It lacks individual channel activity but dynamically affects properties by forming heterotetramers with other isoforms. hERG Protein, Human (HEK293, GFP, His) is the recombinant human-derived hERG protein, expressed by HEK293 , with C-10*His, C-GFP labeled tag.
- Application
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种属
Human
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表达系统
HEK293
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标签
C-10*His;C-GFP
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q12809
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表达区间
M1-S1159, ΔP141-L350 and ΔS871-R1005
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蛋白长度
Full Length
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The human Ether-a-go-go Related Gene (hERG) encodes a voltage-gated potassium channel, I_Kr, which plays a crucial role in cardiac repolarization. Dysregulation of hERG is linked to various cardiovascular diseases, including Long QT syndrome, which can lead to life-threatening arrhythmias. Given its significance in pharmacology, hERG has become a pivotal target in drug development, as many pharmaceutical compounds have been identified to either block or affect hERG function, leading to potential cardiac toxicity. The study of hERG recombinant protein is essential to understanding its biophysical properties, regulatory mechanisms, and interactions with drugs. Recombinant hERG proteins allow for controlled experimental conditions, facilitating detailed investigations into the structure-function relationship of the channel, screening for drug interactions, and elucidating the molecular basis of channelopathies. This area of research is critical not only for improving drug safety profiles but also for designing novel therapeutic agents that selectively modulate hERG activity to mitigate adverse cardiac effects. As the demand for precision medicine increases, understanding hERG's detailed mechanisms through recombinant protein studies is imperative for advancing cardiovascular therapeutics and ensuring patient safety in drug therapies.
