Analytical Data
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基因名
Legumain
- Application
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别名
Legumain; Asparaginyl Endopeptidase; Protease Cysteine 1; LGMN; PRSC1
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种属
Human
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表达系统
HEK293
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标签
C-6*His
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
AAH03061.1
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表达区间
I18-Y433
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氨基酸序列
IPIDDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQIIHRNGIPDEQIVVMMYDDIAYSEDNPTPGIVINRPNGTDVYQGVPKDYTGEDVTPQNFLAVLRGDAEAVKGIGSGKVLKSGPQDHVFIYFTDHGSTGILVFPNEDLHVKDLNETIHYMYKHKMYRKMVFYIEACESGSMMNHLPDNINVYATTAANPRESSYACYYDEKRSTYLGDWYSVNWMEDSDVEDLTKETLHKQYHLVKSHTNTSHVMQYGNKTISTMKVMQFQGMKRKASSPVPLPPVTHLDLTPSPDVPLTIMKRKLMNTNDLEESRQLTEEIQRHLDARHLIEKSVRKIVSLLAASEAEVEQLLSERAPLTGHSCYPEALLHFRTHCFNWHSPTYEYALRHLYVLVNLCEKPYPLHRIKLSMDHVCLGHY
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蛋白长度
Partial
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分子量
55-60 kDa,.
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Legumain, an asparaginyl endopeptidase, is a member of the cysteine protease family and has gained significant attention in the fields of biochemistry and molecular biology due to its unique substrate specificity and potential applications in therapeutic contexts. Its expression is primarily associated with various pathological conditions, including cancer, where it plays a role in tumor progression, immune evasion, and metastasis. Researchers have identified Legumain as a promising target for drug development, particularly in designing selective inhibitors that could combat tumors by modulating its activity. Additionally, Legumain is involved in the processing of several proteins, which further elucidates its importance in cellular functions and signaling pathways. The development of recombinant Legumain has facilitated detailed studies on its structure-function relationships, enabling researchers to unravel the mechanisms underlying its enzymatic activity and substrate interactions. This research not only enhances our understanding of Legumain's biological role but also contributes to the advancement of biotechnological applications, such as the creation of targeted drug delivery systems and diagnostic tools. With ongoing advancements in recombinant protein engineering, the characterization and manipulation of Legumain hold promise for innovative therapeutic strategies in cancer treatment and other diseases where this protease plays a critical role.
