Analytical Data
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基因名
Phospholipase C
- Application
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别名
BC_0670; Phosphatidylcholine cholinephosphohydrolase; Phospholipase C
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种属
Others
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表达系统
E. coli
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标签
Tag Free
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q81HW1
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表达区间
M1-R283
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蛋白长度
Full Length
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分子量
30.7 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Phospholipase C (PLC) is an essential enzyme that plays a critical role in cellular signaling pathways by hydrolyzing phosphatidylinositol 4,5-bisphosphate (PIP2) to generate inositol trisphosphate (IP3) and diacylglycerol (DAG). These two second messengers are pivotal for various physiological processes, including cell proliferation, differentiation, and apoptosis. Given its significance, PLC has garnered considerable attention in biomedical research, particularly in the context of understanding its implications in diseases such as cancer, cardiovascular disorders, and neurological conditions. The recombinant production of PLC proteins has enabled researchers to study their structure-function relationships more thoroughly and to explore their activity in various cellular contexts. The advancements in recombinant DNA technology and protein expression systems have facilitated the generation of active PLC proteins, providing a platform for detailed biochemical assays, structural studies, and potential therapeutic applications. By utilizing these recombinant proteins, researchers aim to elucidate the mechanisms of PLC activation, regulation, and its downstream signaling pathways, contributing to our understanding of cell biology and the development of targeted therapeutic strategies.
