Analytical Data
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Gene name
AMSH-LP
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简介
AMSH-LP is a zinc metalloprotease that selectively cleaves "Lys-63" linked polyubiquitin chains and acts as a positive regulator of the TORC1 signaling pathway. It specifically deubiquitinates SESN2, disrupts its interaction with the GATOR2 complex and inhibits SESN2-mediated regulation of TORC1. AMSH-LP Protein, Human is the recombinant human-derived AMSH-LP protein, expressed by E. coli , with tag free.
- Application
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Alternative Names
STAMBPL1; AMSH-like protease; AMSH-LP; STAM-binding protein-like 1
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Species
Human
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Source
E. coli
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Tag
Tag Free
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q96FJ0
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Expression Region
D2-R436
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Protein Length
Partial
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Molecular Weight
49.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The study of the AMSH-LP protein, an essential member of the AMSH (Associated Maturation of Endosomal Sorting Complex Required for Transport) family, has gained significant attention due to its vital role in the regulation of protein degradation and cell signaling pathways. AMSH-LP is known for its deubiquitinating activity, which contributes to the control of various cellular processes, including the modulation of the endosomal sorting pathway. Ubiquitination and deubiquitination are critical in maintaining cellular homeostasis, influencing the stability and activity of numerous proteins. Disruptions in these processes can lead to various diseases, including cancer and neurodegenerative disorders. Research on AMSH-LP has revealed its involvement in several key pathways, making it a potential target for therapeutic interventions. Understanding the structural and functional aspects of AMSH-LP is crucial for elucidating its mechanisms of action and identifying its role in disease states. The ongoing investigation into its interactions with other proteins and cellular components could provide insights into its potential as a biomarker for disease progression or a target for drug development. Thus, the research surrounding AMSH-LP not only enhances our fundamental understanding of cellular regulation but also opens avenues for innovative therapeutic strategies in treating diseases associated with protein mismanagement.
