Analytical Data
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Gene name
OTUD7B
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简介
The OTUD7B protein acts as a negative regulator to inhibit the non-canonical NF-kappa-B pathway by deubiquitinating TRAF3, thereby inhibiting B cell responses. It prevents degradation of “Lys-48”-linked polyubiquitin chains on TRAF3 during atypical NF-kappa-B stimulation, avoiding overactivation of the pathway and affecting mucosal immunity. OTUD7B Protein, Human (sf9) is the recombinant human-derived OTUD7B protein, expressed by sf9 insect cells , with tag free.
- Application
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Alternative Names
OTUD7B; OTU domain-containing protein 7B; Cellular zinc finger anti-NF-kappa-B protein; Cezanne; Zinc finger A20 domain-containing protein 1; Zinc finger protein Cezanne
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Species
Human
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Source
Baculovirus
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Tag
Tag Free
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q6GQQ9
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Expression Region
T2-F843
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Protein Length
Partial
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Molecular Weight
92.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
OTUD7B, a member of the ovarian tumor domain-containing deubiquitinating enzyme family, has garnered considerable interest in the field of molecular biology and cancer research due to its role in regulating protein ubiquitination, a crucial post-translational modification that influences various cellular processes, including signal transduction, cell cycle progression, and apoptosis. Dysregulation of ubiquitin pathways is often implicated in cancer development and progression, making OTUD7B a potential therapeutic target. Studies have demonstrated that OTUD7B can remove ubiquitin chains from substrate proteins, thereby stabilizing key regulatory proteins and modulating cellular responses to stress and inflammation. Recent research has unveiled its involvement in the regulation of immune responses and its potential role in the pathogenesis of several diseases, including cancers such as breast and lung cancer. Understanding the enzymatic activity and substrate specificity of OTUD7B is essential for elucidating its functional significance in cellular homeostasis and disease. Consequently, the development of OTUD7B recombinant proteins has emerged as a crucial tool for investigating its molecular mechanisms and exploring its therapeutic potential, paving the way for groundbreaking advances in targeted cancer therapies and biomarker discovery. This research not only enhances our understanding of OTUD7B's biological functions but also contributes to the broader knowledge of the ubiquitin-proteasome system in health and disease.
