Recombinant Human USP6 Protein (Baculovirus)，GST

USP6

USP6 protein is an ATP-independent isopeptidase with unique deubiquitinase activity that can cleave the C-terminus of ubiquitin and catalyze its own deubiquitination. Notably, it coordinates ARF6 plasma membrane localization and selectively affects ARF6-dependent endocytic protein trafficking. USP6 Protein, Human (sf9, GST) is the recombinant human-derived USP6 protein, expressed by sf9 insect cells , with N-GST labeled tag.

SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

The fundamental role of USP6 is specific removal of ubiquitin from substrates. USP6 catalyses the ubiquitin from the substrate Ub-Rho110 to release fluorophores. Rho110 will release 535 nM emission light under the excitation condition of 485 nM. The signal of which can be quickly and reliably captured using a microplate reader. At a concentration of 0.03 nM, USP6 reacts with the Ub-Rho110 substrate. The signal window after half an hour of reaction can be greater than or equal to 10.

USP6; Ubiquitin carboxyl-terminal hydrolase 6; Deubiquitinating enzyme 6; Proto-oncogene TRE-2; Ubiquitin thioesterase 6; Ubiquitin-specific-processing protease 6

Human

Baculovirus

N-GST

Greater than 90% as determined by SDS-PAGE.

P35125

K529-Q1406

Partial

125.1 KDa

< 1.0 EU per μg protein as determined by the LAL method.

Freeze-dried powder

PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.