Recombinant Human UCHL5 Protein

UCHL5

UCHL5 is a highly specific protease that acts as a deubiquitinating enzyme intricately linked to the 19S proteasome subunit, cleaving "Lys-48" linked polyubiquitin chains. Although the INO80 complex is inert, transient interactions between INO80 and the proteasome (facilitated by ADRM1) activate UCHL5. UCHL5 Protein, Human is the recombinant human-derived UCHL5 protein, expressed by E. coli , with tag free.

SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

UCHL5; Ubiquitin carboxyl-terminal hydrolase isozyme L5; UCH-L5; Ubiquitin C-terminal hydrolase UCH37; Ubiquitin thioesterase L5

Human

E. coli

Tag Free

Greater than 90% as determined by SDS-PAGE.

Q9Y5K5

T2-K329

Partial

37.6 KDa

< 1.0 EU per μg protein as determined by the LAL method.

Freeze-dried powder

PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.