Analytical Data
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基因名
BMP-4
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简介
Bone morphogenetic protein 4 (BMP-4) is a polymorphic ligand protein belonging to the TGF-β family, which is involved in the circulation of the vascular system and can activate receptors on vascular cells[1]. BMP-4 binds to type I receptors (ALK-2/-3/-6) and type II receptors (BMPR2, ACVR2A)[2] to increase plaque formation and promote oxidative stress, endothelial dysfunction, and osteogenic differentiation through its pro-inflammatory and pro-atherogenic effects[3]. BMP-4 Protein, Human has a total length of 116 amino acids (S293-R408), is expressed in E. coli cells with tag free.
- Application
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生物活性
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells.The ED50 for this effect is < 60 ng/mL. Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells.The ED50 for this effect is 0.9255 ng/mL, corresponding to a specific activity is 1.08×106 U/mg.
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别名
rHuBMP-4; BMP-2B
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种属
Human
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表达系统
E. coli
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标签
Tag Free
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纯度
Greater than 95% as determined by reducing SDS-PAGE.
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蛋白编号
P12644
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表达区间
S293-R408
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蛋白长度
Full Length of Mature Protein
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分子量
13 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
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Protein Description
Bone Morphogenetic Protein 4 (BMP-4) is a member of the transforming growth factor-beta (TGF-β) superfamily, known for its crucial role in regulating embryonic development, particularly in bone and cartilage formation. Research on BMP-4 has gained momentum due to its significant implications in various biological processes, including mesenchymal stem cell differentiation, tissue regeneration, and repair, as well as its involvement in pathological conditions like cancer and degenerative diseases. BMP-4 functions primarily through activating specific receptors on target cells, leading to a cascade of intracellular signaling pathways that influence gene expression. The recombinant production of BMP-4 has facilitated extensive studies aimed at understanding its mechanism of action and therapeutic potential. Researchers have developed various recombinant forms of BMP-4, enabling investigations into its precise effects on skeletal tissues and its utility in clinical applications, such as enhancing bone healing in orthopedic surgeries and treating bone-related disorders. Furthermore, studies have delved into the molecular interactions of BMP-4 with other signaling pathways, underscoring its role in a complex network of growth factors. Overall, the ongoing exploration of BMP-4, particularly its recombinant protein forms, continues to shed light on its biological significance and pave the way for innovative therapeutic strategies in regenerative medicine.
