Analytical Data
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Gene name
IL-23R
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简介
The IL-23R protein is critical in immune responses by binding to IL12RB1 to form the interleukin 23 receptor. It binds to IL23 and activates T cells, NK cells and potentially macrophages/myeloid cells through Jak-Stat signaling. IL-23R Protein, Human (HEK293, mFc) is the recombinant human-derived IL-23R protein, expressed by HEK293 , with C-mFc labeled tag.
- Application
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Biological Activity
1.Immobilized Human IL-23 alpha&Mouse IL-12, His Tag at 5 μg/mL (100 μl/Well) on the plate. Dose response curve for Human IL-23R, mFc Tag with the EC50 of 0.33 μg/mL determined by ELISA. 2.Immobilized Biotinylated Human IL-23 alpha&IL-12 beta, His Tag at 2 μg/mL (100 μl/well) on the streptavidin precoated plate (5 μg/mL). Dose response curve for Human IL-23R, mFc Tag with the EC50 of 11.1 ng/mL determined by ELISA. 3. Immobilized Biotinylated Human IL-23 alpha&IL 12 beta, His Tag at 5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human IL-23R, mFc Tag with the EC50 of 37.0ng/ml determined by ELISA.
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Alternative Names
IBD17; IL-23 R; IL-23 receptor
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Species
Human
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Source
HEK293
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Tag
C-mFc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q5VWK5-1
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Expression Region
G24-G355
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Protein Length
Extracellular Domain
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Molecular Weight
80-115 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Interleukin-23 receptor (IL-23R) is a critical component of the immune system, playing a significant role in the regulation of inflammatory responses. It primarily binds to interleukin-23 (IL-23), a cytokine involved in the differentiation and proliferation of T-helper 17 (Th17) cells, which are crucial in various autoimmune diseases, including psoriasis, inflammatory bowel disease, and multiple sclerosis. Studies have shown that genetic polymorphisms in the IL-23R gene are associated with an increased susceptibility to these conditions, highlighting its importance as a therapeutic target. The production of recombinant IL-23R protein can provide valuable insights into its structure and function, enabling researchers to explore its signaling pathways and interactions with IL-23. This recombinant protein can be used in various applications, including drug development and the creation of targeted therapies that modulate immune responses. Understanding the role of IL-23R in chronic inflammation and autoimmune disorders could lead to the development of novel therapeutic strategies aimed at mitigating these conditions, thereby improving patient outcomes.
