Analytical Data
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基因名
Calreticulin-3/CALR3
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简介
Calreticulin-3/CALR3 Protein, Human is a recombinant human Calreticulin-3 expressed in E. coli. Calreticulin (CRT) is a highly conserved chaperone-like lectin that regulates Ca2+ homeostasis and participates in protein quality control in the endoplasmic reticulum (ER)[1].
- Application
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别名
rHuCalreticulin-3; Calreticulin-3; calreticulin-2; calsperin; CALR3; CRT2
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种属
Human
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表达系统
E. coli
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标签
Tag Free
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q96L12
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表达区间
T20-L384
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氨基酸序列
TVYFQEEFLDGEHWRNRWLQSTNDSRFGHFRLSSGKFYGHKEKDKGLQTTQNGRFYAISARFKPFSNKGKTLVIQYTVKHEQKMDCGGGYIKVFPADIDQKNLNGKSQYYIMFGPDICGFDIKKVHVILHFKNKYHENKKLIRCKVDGFTHLYTLILRPDLSYDVKIDGQSIESGSIEYDWNLTSLKKETSPAESKDWEQTKDNKAQDWEKHFLDASTSKQSDWNGDLDGDWPAPMLQKPPYQDGLKPEGIHKDVWLHRKMKNTDYLTQYDLSEFENIGAIGLELWQVRSGTIFDNFLITDDEEYADNFGKATWGETKGPEREMDAIQAKEEMKKAREEEEEELLSGKINRHEHYFNQFHRRNEL
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蛋白长度
Full Length of Mature Protein
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分子量
40-50 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Calreticulin-3 (CALR3) is a member of the calreticulin family, which is characterized by its role in calcium binding and regulation within the endoplasmic reticulum. Its functions extend beyond calcium homeostasis; CALR3 is also involved in various cellular processes including cell adhesion, immune responses, and chaperone activity, thereby influencing cell signaling and survival. Research has shown that CALR3 plays a critical role in the modulation of immune responses, particularly in the context of cancer, where its expression levels may correlate with tumor progression and patient prognosis. This has sparked interest in developing CALR3 as a potential biomarker for cancer diagnosis and therapy. Moreover, the study of recombinant CALR3 proteins offers insights into their structural and functional properties, providing opportunities for therapeutic applications. Structural studies reveal how CALR3 interacts with other proteins and its role in calcium signaling pathways. The generation of recombinant CALR3 proteins allows for detailed investigation of these interactions, paving the way for therapeutic strategies that target CALR3-related pathways in diseases. Understanding the molecular mechanisms by which CALR3 influences cell behavior has significant implications for cancer research and therapy, making it a key target for further scientific exploration.
