Analytical Data
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Gene name
OCAM/NCAM2
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简介
OCAM/NCAM2 proteins are potential key players in selective fasciculations and interregional projections in olfactory axons. Its specific mechanism and functional significance in guiding axonal development remain to be further elucidated. OCAM/NCAM2 Protein, Human (HEK293, His) is the recombinant human-derived OCAM/NCAM2 protein, expressed by HEK293 , with C-His labeled tag.
- Application
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Biological Activity
Measured by the ability of the immobilized protein to support the adhesion of MCF-7 human breast cancer cells. The ED50 for this effect is 0.5143 μg/mL, corresponding to a specific activity is 1944.39 units/mg. Measured by the ability of the immobilized protein to support the adhesion of MCF-7 human breast cancer cells. The ED50 for this effect is 0.5143 μg/mL，corresponding to a specific activity is 1944.39 units/mg.
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Alternative Names
Neural cell adhesion molecule 2; NCAM-2; NCAM21
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Species
Human
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Source
HEK293
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Tag
C-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O15394-1
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Expression Region
L20-N697
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Protein Length
Extracellular Domain
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Molecular Weight
90.1 kDa.
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
OCAM (Oligodendrocyte Cell Adhesion Molecule) and NCAM2 (Neural Cell Adhesion Molecule 2) are important members of the immunoglobulin superfamily, playing crucial roles in cell adhesion, neural development, and myelination processes. Research into OCAM/NCAM2 recombinant proteins has gained momentum due to their implications in neurobiology and potential therapeutic applications in neurodegenerative diseases and demyelinating disorders. OCAM, primarily expressed in oligodendrocytes, is involved in the maintenance of myelin integrity and the interaction of oligodendrocytes with axons, while NCAM2, expressed in various neural tissues, is essential for neuronal plasticity and synaptic function. Understanding the structural and functional characteristics of these adhesion molecules could provide insights into their mechanisms of action and their contributions to neuronal network formation. Moreover, abnormalities in their expression or function have been linked to conditions such as multiple sclerosis, schizophrenia, and other neurological disorders. Therefore, studying OCAM/NCAM2 recombinant proteins not only enhances our understanding of neural development and function but also opens avenues for novel therapeutic strategies targeting these adhesion molecules in neurodegenerative and psychiatric conditions. The development of these recombinant proteins allows for precise examination of their roles and interactions, facilitating advancements in both basic and applied neuroscience research.
