Analytical Data
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Gene name
Nicastrin
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简介
Nicastrin is an important subunit of the γ-secretase complex and plays a key role in the endoproteolytic cleavage of integral membrane proteins, including Notch receptors and amyloid-β precursor protein (APP). In the γ-secretase complex, nicastin acts as a key regulator of Notch and Wnt signaling cascades and affects downstream processes. Nicastrin Protein, Mouse (HEK293, hFc) is the recombinant mouse-derived Nicastrin protein, expressed by HEK293 , with C-hFc labeled tag.
- Application
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Alternative Names
Nicastrin; NCSTN; KIAA0253
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Species
Mouse
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Source
HEK293
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Tag
C-hFc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P57716
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Expression Region
G28-E668
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Protein Length
Lumenal Domain
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Molecular Weight
117 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Nicastrin is a critical component of the gamma-secretase complex, an intramembrane protease that plays a vital role in the processing of various membrane proteins, including the amyloid precursor protein (APP). This complex is particularly significant in Alzheimer's disease research, as the cleavage of APP by gamma-secretase leads to the production of amyloid-beta peptides, which aggregate to form plaques associated with neurodegeneration. The study of nicastrin, therefore, has garnered attention due to its involvement in regulating the activity of gamma-secretase and its potential implications in therapeutic interventions for Alzheimer's disease. Researchers aim to elucidate the structure and function of nicastrin, exploring its interactions within the gamma-secretase complex to understand its role in the cleavage of substrates. Additionally, recombinant nicastrin proteins are being produced for functional assays and structural studies, which may aid in the development of drugs that can modulate gamma-secretase activity. By targeting nicastrin and its interactions, scientists hope to inhibit the formation of harmful amyloid-beta peptides, offering new avenues for treatment in Alzheimer's disease and other cognitive disorders. This research continues to expand our understanding of gamma-secretase and its components, highlighting the importance of nicastrin in both basic neuroscience and clinical applications.
