Analytical Data
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Gene name
pfHPRT
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简介
The pfHPRT (Plasmodium falciparum hypoxanthine-guanine phosphoribosyltransferase) protein is a member of the LDH/MDH (lactate dehydrogenase/malate dehydrogenase) superfamily. It plays a crucial role in the purine metabolism salvage pathway of the malaria-causing parasite Plasmodium falciparum. pfHPRT Protein, P. falciparum (His) is the recombinant pfHPRT protein, expressed by E. coli , with N-His labeled tag.
- Application
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Alternative Names
L-lactate dehydrogenase; PF3D7_1324900
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Species
Others
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q76NM3
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Expression Region
M1-L231
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Protein Length
Partial
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The phosphoribosyltransferase enzyme, specifically the phosphoribosyltransferase encoded by the HPRT gene (Hypoxanthine-guanine phosphoribosyltransferase, pfHPRT), plays a crucial role in purine metabolism. This enzyme facilitates the salvage pathway, allowing the recycling of hypoxanthine and guanine to generate nucleotides, which are essential for DNA and RNA synthesis. Mutations in the HPRT gene can lead to Lesch-Nyhan syndrome, characterized by neurological and behavioral abnormalities, highlighting the importance of this enzyme in human health. As such, the recombinant production of pfHPRT has garnered significant interest for both therapeutic and research applications. The study of pfHPRT not only helps elucidate the biochemical pathways involved in purine metabolism but also aids in the development of potential treatments for disorders caused by HPRT deficiency. Researchers aim to produce and characterize the recombinant protein to understand its structure-function relationship and investigate its potential as a target for drug development. Furthermore, the successful expression and purification of pfHPRT can pave the way for innovative strategies in gene therapy, where restoring normal enzyme function may alleviate the symptoms associated with HPRT-related disorders. Therefore, the exploration of pfHPRT as a recombinant protein serves as a pivotal foundation for advancing our understanding of metabolic diseases and developing new therapeutic approaches.
