Cat: IPD-X23479

Recombinant Human C-Reactive Protein,His

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Analytical Data

  • Gene name

    C-Reactive

  • 简介

    C-Reactive Protein, an acute-phase protein, is a marker of inflammation and is produced by the liver. It is used as a diagnostic tool to assess the presence and severity of inflammation in various diseases. C-Reactive Protein's role in inflammation detection and its clinical significance in disease management make it a subject of interest in medical research. C-Reactive Protein, Human (His) is the recombinant human-derived C-Reactive protein, expressed by E. coli , with C-6*His labeled tag.

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Biological Activity

    1.Immobilized Human CRP-His at 10 μg/mL (100 μl/well) can bind Human CD32a-His Biotinylated by NHS-biotin prior to testing.The ED50 is 7.64 μg/mL. 2.Measured by its binding ability in a functional ELISA. Immobilized Human CD32a at 2 μg/mL (100 μL/well) can bind Biotinylated Human C-Reactive. The ED50 for this effect is 1-5 μg/mL. Measured by its binding ability in a functional ELISA. Immobilized Human CD32a at 2 μg/mL (100 μL/well) can bind Biotinylated Human C-Reactive. The ED50 for this effect is 1.182 μg/mL.

  • Alternative Names

    rHuC-reactive protein/CRP, His; C-Reactive Protein; CRP; PTX1

  • Species

    Human

  • Source

    E. coli

  • Tag

    C-6*His

  • Purity

    Greater than 95% as determined by SDS-PAGE.

  • Uniprot

    P02741-1

  • Expression Region

    Q19-P224

  • AA Sequence

    QTDMSRKAFVFPKESDTSYVSLKAPLTKPLKAFTVCLHFYTELSSTRGYSIFSYATKRQDNEILIFWSKDIGYSFTVGGSEILFEVPEVTVAPVHICTSWESASGIVEFWVDGKPRVRKSLKKGYTVGAEASIILGQEQDSFGGNFEGSQSLVGDIGNVNMWDFVLSPDEINTIYLGGPFSPNVLNWRALKYEVQGEVFTKPQLWP

  • Protein Length

    Full Length of Isoform-1 Mature Protein

  • Molecular Weight

    27 kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

Quality inspection process

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Protein Description

CRHBP, or Corticotropin-Releasing Hormone Binding Protein, is a pivotal component in the neuroendocrine stress response, acting primarily as a regulator of corticotropin-releasing hormone (CRH) levels. The CRH system influences various physiological processes, including stress response, immune function, and metabolic regulation. Dysregulation of this system has been implicated in various psychiatric disorders, such as depression and anxiety, as well as in obesity and metabolic syndrome. Research into the structure and function of CRHBP has gained attention due to its potential role as a therapeutic target. Recombinant CRHBP, produced through genetic engineering techniques, provides a valuable tool for studying the protein's interactions with CRH and other ligands, allowing for a deeper understanding of its biological functions. Furthermore, elucidating the mechanisms by which CRHBP modulates stress response pathways could pave the way for the development of novel treatments for stress-related disorders. Investigating the properties of recombinant CRHBP may also unveil its potential as a biomarker for assessing stress levels and related health issues, thereby supporting the development of personalized medicine approaches. Overall, the research on recombinant CRHBP serves as a critical foundation for advancing our knowledge of the neuroendocrine system and addressing the challenges posed by stress-related conditions.

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