Analytical Data
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Gene name
S100A8
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简介
S100A8 consists of calcium and zinc bound S100A8, which plays a critical regulatory role in inflammation and immune responses. As calprotectin, it contributes to leukocyte function, regulates the cytoskeleton, and activates intracellular NADPH oxidase. S100A8 Protein, Human (N-His) is the recombinant human-derived S100A8 protein, expressed by E. coli , with N-6*His labeled tag.
- Application
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Alternative Names
Protein S100-A8; S100A8; Calgranulin-A; Cystic fibrosis antigen; Leukocyte L1 complex light chain; MRP-8
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Species
Human
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Source
E. coli
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Tag
N-6*His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
P05109
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Expression Region
M1-E93
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Protein Length
Full Length
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Molecular Weight
12-13 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
S100A8, a member of the S100 protein family, is a calcium-binding protein predominantly expressed in myeloid cells, playing a pivotal role in various physiological and pathological processes. Understanding S100A8 has garnered significant interest due to its involvement in inflammation, cancer progression, and autoimmune diseases. The protein functions by influencing the immune response, acting as a damage-associated molecular pattern (DAMP) that promotes inflammatory signaling pathways through receptor interactions, notably with TLR4 and RAGE. Elevated levels of S100A8 have been linked to various inflammatory conditions, such as rheumatoid arthritis and inflammatory bowel disease, highlighting its potential as a biomarker and therapeutic target. Additionally, research has indicated that S100A8 may contribute to tumor microenvironment modifications, promoting cancer cell proliferation and metastasis. The recombinant protein has been utilized in numerous studies to dissect its function, binding properties, and therapeutic implications. As researchers continue to explore the multifaceted roles of S100A8, the production and study of recombinant S100A8 proteins enhance our understanding of its biological functions and the mechanisms underlying its involvement in disease, paving the way for novel therapeutic strategies to modulate its activity in relevant pathologies.
