Analytical Data
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基因名
TCN1
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简介
The TCN1 protein is critical for mitochondrial processes and binds to the mitochondrial light chain promoter to regulate mitochondrial transcription. As a key component of the mitochondrial transcription initiation complex (including TFB2M and POLRMT), TCN1 plays a crucial role in the basal transcription of mitochondrial DNA. TCN1 Protein, Human (P.pastoris, His) is the recombinant human-derived TCN1 protein, expressed by P. pastoris , with N-His labeled tag.
- Application
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别名
Haptocorin; Haptocorrin; HC; Transcobalamin 1; Vitamin B12 binding protein; Vitamin B12 binding protein R binder family
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种属
Human
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表达系统
P. pastoris
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标签
N-His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P20061
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表达区间
E24-Y433
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氨基酸序列
EICEVSEENYIRLKPLLNTMIQSNYNRGTSAVNVVLSLKLVGIQIQTLMQKMIQQIKYNVKSRLSDVSSGELALIILALGVCRNAEENLIYDYHLIDKLENKFQAEIENMEAHNGTPLTNYYQLSLDVLALCLFNGNYSTAEVVNHFTPENKNYYFGSQFSVDTGAMAVLALTCVKKSLINGQIKADEGSLKNISIYTKSLVEKILSEKKENGLIGNTFSTGEAMQALFVSSDYYNENDWNCQQTLNTVLTEISQGAFSNPNAAAQVLPALMGKTFLDINKDSSCVSASGNFNISADEPITVTPPDSQSYISVNYSVRINETYFTNVTVLNGSVFLSVMEKAQKMNDTIFGFTMEERSWGPYITCIQGLCANNNDRTYWELLSGGEPLSQGAGSYVVRNGENLEVRWSKY
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蛋白长度
Full Length of Mature Protein
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
TCN1 (T-complex protein 1 subunit gamma) is a member of the chaperonin-containing TCP1 (CCT) complex, which plays a crucial role in the proper folding of newly synthesized proteins, particularly those involved in cellular stress responses and cytoskeletal assembly. The importance of TCN1 in cellular function is underscored by its involvement in essential biological processes, such as signal transduction, cell motility, and development. Mutations or dysregulation of TCN1 have been implicated in various diseases, including cancer and neurodegenerative disorders, making it a target of interest for therapeutic interventions. Thus, the study of recombinant TCN1 protein is essential for understanding its structure-function relationships and the molecular mechanisms of its action. Recombinant protein studies facilitate the investigation of TCN1's chaperone activities, its interactions with other proteins, and its role in the assembly of multi-subunit complexes. By producing TCN1 in a recombinant system, researchers can obtain large quantities of pure protein for in vitro assays, structural biology studies, and potential drug screening applications. Furthermore, uncovering the functional properties of TCN1 may reveal novel insights into the development of targeted therapies and provide valuable information for the design of interventions aimed at correcting the misfolding of proteins linked to various diseases. Through a better understanding of TCN1's biochemical properties and biological significance, researchers aim to elucidate its contribution to cellular homeostasis and the pathogenesis of diseases associated with protein misfolding and aggregation.
