Analytical Data
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Gene name
Galectin-3/LGALS3
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简介
Galectin-3/LGALS3 Protein is a type of galactoside-binding lectin that has a high binding affinity for carbohydrates with β-galactoside linkages. Galectin-3/LGALS3 Protein interacts with glycosylated proteins to mediate intercellular interactions and adhesion to the extracellular matrix. Galectin-3/LGALS3 Protein plays various roles, including regulating signaling pathways such as Wnt/β-catenin, affecting cell proliferation and survival, modulating immune functions, and influencing tumor progression. Galectin-3/LGALS3 Protein, Human is a recombinant galectin-3/LGALS3 protein expressed in E. coli[1][2].
- Application
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Biological Activity
Measured by the ability of the immobilized protein to support the adhesion of MOLT-4 human acute lymphoblastic leukemia cells. The ED50 for this effect is 1-3 μg/mL. Measured by its ability to induce IL-8 secretion in HT1080 human fibrosarcoma cells transfected with human OX40/TNFRSF4. The ED50 for this effect is 3.527ng/mL, corresponding to a specific activity is 2.836×105 U/mg.
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Species
Human
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Source
E. coli
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Tag
Tag Free
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
P17931
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Expression Region
A2-I250
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AA Sequence
ADNFSLHDALSGSGNPNPQGWPGAWGNQPAGAGGYPGASYPGAYPGQAPPGAYPGQAPPGAYPGAPGAYPGAPAPGVYPGPPSGPGAYPSSGQPSATGAYPATGPYGAPAGPLIVPYNLPLPGGVVPRMLITILGTVKPNANRIALDFQRGNDVAFHFNPRFNENNRRVIVCNTKLDNNWGREERQSVFPFESGKPFKIQVLVEPDHFKVAVNDAHLLQYNHRVKKLNEISKLGISGDIDLTSASYTMI
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Protein Length
Full Length of Mature Protein
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Molecular Weight
29 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Galectin-3, also known as LGALS3, is a member of the galectin family of proteins characterized by their ability to bind β-galactosides. It plays a crucial role in various biological processes, including cell adhesion, immune response modulation, and apoptosis. Research has increasingly focused on Galectin-3 due to its involvement in numerous pathological conditions, such as cancer, cardiovascular diseases, and fibrosis. Elevated levels of Galectin-3 have been associated with poor prognoses in various malignancies, highlighting its potential as a biomarker for cancer diagnosis and treatment. Additionally, Galectin-3 is implicated in the progression of heart failure and chronic diseases, making it a target for therapeutic interventions. The recombinant expression of LGALS3 enables detailed studies of its molecular functions and interactions, facilitating the exploration of its role in disease mechanisms and the development of Galectin-3 inhibitors for therapeutic applications. Thus, understanding the biological functions of recombinant Galectin-3 can provide insights into its potential as a biomarker and therapeutic target, which may lead to novel strategies for the diagnosis and treatment of diseases.
