Analytical Data
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Gene name
WDR77
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简介
WDR77 is a non-catalytic component of the methylosome complex with PRMT5 and CLNS1A and regulates arginine to dimethylarginine in spliceosomal Sm proteins and histones. This modification directs Sm proteins to SMN complexes, helping them assemble into small nuclear ribonucleoprotein core particles. WDR77 Protein, Human (His-SUMO) is the recombinant human-derived WDR77 protein, expressed by E. coli , with N-His, N-SUMO labeled tag.
- Application
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Alternative Names
2610312E17Rik; Androgen receptor cofactor p44; C79984; HKMT1069; MEP 50; MEP-50; MEP50; Methylosome protein 50; WDR77
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Species
Human
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Source
E. coli
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Tag
N-His;N-SUMO
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9BQA1
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Expression Region
M1-L312
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AA Sequence
MRKETPPPLVPPAAREWNLPPNAPACMERQLEAARYRSDGALLLGASSLSGRCWAGSLWLFKDPCAAPNEGFCSAGVQTEAGVADLTWVGERGILVASDSGAVELWELDENETLIVSKFCKYEHDDIVSTVSVLSSGTQAVSGSKDICIKVWDLAQQVVLSSYRAHAAQVTCVAASPHKDSVFLSCSEDNRILLWDTRCPKPASQIGCSAPGYLPTSLAWHPQQSEVFVFGDENGTVSLVDTKSTSCVLSSAVHSQCVTGLVFSPHSVPFLASLSEDCSLAVLDSSLSELFRSQAHRDFVRDATWSPLNHSL
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Protein Length
Partial
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Molecular Weight
51 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
WDR77, or WD repeat-containing protein 77, is a multifunctional protein that plays a significant role in various cellular processes, including gene regulation and RNA splicing. It contains several WD-repeat motifs, which are commonly involved in protein-protein interactions, suggesting its involvement in forming protein complexes that regulate transcription and signal transduction pathways. Research indicates that WDR77 is essential for the proper functioning of the thyroid-stimulating hormone (TSH) signaling pathway, influencing thyroid hormone synthesis and metabolism. Additionally, aberrations in WDR77 expression and function have been linked to several diseases, including cancer and developmental disorders. Recent studies have focused on the reconstitution of WDR77 in vitro to better understand its structural and functional properties, as well as its interactions with other proteins. This research not only provides insight into the unique roles WDR77 plays within the cell but also holds potential for therapeutic applications, particularly in diseases where this protein is dysregulated. Understanding the mechanisms underlying WDR77's function could pave the way for novel strategies in treating related pathologies. As such, ongoing investigations are aimed at elucidating the precise biochemical pathways in which WDR77 is involved, the impact of post-translational modifications on its activity, and the implications of its interactions with other key regulatory proteins within the cell.
