Analytical Data
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基因名
WDR77
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简介
WDR77 is a non-catalytic component of the methylosome complex with PRMT5 and CLNS1A and regulates arginine to dimethylarginine in spliceosomal Sm proteins and histones. This modification directs Sm proteins to SMN complexes, helping them assemble into small nuclear ribonucleoprotein core particles. WDR77 Protein, Human (His-SUMO) is the recombinant human-derived WDR77 protein, expressed by E. coli , with N-His, N-SUMO labeled tag.
- Application
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别名
2610312E17Rik; Androgen receptor cofactor p44; C79984; HKMT1069; MEP 50; MEP-50; MEP50; Methylosome protein 50; WDR77
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种属
Human
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表达系统
E. coli
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标签
N-His;N-SUMO
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9BQA1
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表达区间
M1-L312
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氨基酸序列
MRKETPPPLVPPAAREWNLPPNAPACMERQLEAARYRSDGALLLGASSLSGRCWAGSLWLFKDPCAAPNEGFCSAGVQTEAGVADLTWVGERGILVASDSGAVELWELDENETLIVSKFCKYEHDDIVSTVSVLSSGTQAVSGSKDICIKVWDLAQQVVLSSYRAHAAQVTCVAASPHKDSVFLSCSEDNRILLWDTRCPKPASQIGCSAPGYLPTSLAWHPQQSEVFVFGDENGTVSLVDTKSTSCVLSSAVHSQCVTGLVFSPHSVPFLASLSEDCSLAVLDSSLSELFRSQAHRDFVRDATWSPLNHSL
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蛋白长度
Partial
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分子量
51 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
WDR77, or WD repeat-containing protein 77, is a multifunctional protein that plays a significant role in various cellular processes, including gene regulation and RNA splicing. It contains several WD-repeat motifs, which are commonly involved in protein-protein interactions, suggesting its involvement in forming protein complexes that regulate transcription and signal transduction pathways. Research indicates that WDR77 is essential for the proper functioning of the thyroid-stimulating hormone (TSH) signaling pathway, influencing thyroid hormone synthesis and metabolism. Additionally, aberrations in WDR77 expression and function have been linked to several diseases, including cancer and developmental disorders. Recent studies have focused on the reconstitution of WDR77 in vitro to better understand its structural and functional properties, as well as its interactions with other proteins. This research not only provides insight into the unique roles WDR77 plays within the cell but also holds potential for therapeutic applications, particularly in diseases where this protein is dysregulated. Understanding the mechanisms underlying WDR77's function could pave the way for novel strategies in treating related pathologies. As such, ongoing investigations are aimed at elucidating the precise biochemical pathways in which WDR77 is involved, the impact of post-translational modifications on its activity, and the implications of its interactions with other key regulatory proteins within the cell.
