Analytical Data
-
Gene name
TsaE
-
简介
The TsaE protein is essential for the generation of the threonylcarbamoyl group at adenosine position 37 (t(6)A37) in the tRNA that decodes the adenine start codon. It may facilitate the transfer of the threoncarbamoyl moiety from threoncarbamoyl-AMP (TC-AMP) to the N6 group of A37, acting synergistically with TsaD and TsaB. TsaE Protein, E.coli is the recombinant E. coli-derived TsaE protein, expressed by E. coli , with tag free.
- Application
-
Alternative Names
tsaE; yjeE; b4168; JW4126tRNA threonylcarbamoyladenosine biosynthesis protein TsaE; t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaE
-
Species
E.coli
-
Source
E. coli
-
Tag
Tag Free
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
P0AF67
-
Expression Region
M1-G153
-
Protein Length
Full Length
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
TsaE, a protein involved in bacterial cell envelope biosynthesis, has garnered significant attention in microbiological research due to its essential role in maintaining cell integrity and viability in various pathogenic bacteria. As a component of the translocon, TsaE participates in the transport of lipopolysaccharides (LPS) and other critical molecules across the inner membrane, influencing the overall structure and function of the bacterial outer membrane. Given the increasing global concern over antibiotic resistance, understanding the mechanisms governing bacterial cell envelope integrity can provide valuable insights for developing novel therapeutic strategies. Additionally, TsaE has potential applications in biotechnology, particularly in the engineering of attenuated strains for vaccine development or in drug delivery systems. Recent advances in recombinant DNA technology have enabled researchers to produce and characterize TsaE in vitro, paving the way for detailed functional studies and structural analyses. These investigations aim to elucidate the protein’s biochemical properties and interactions within the cell, further contributing to our understanding of bacterial physiology and pathogenesis. By examining TsaE's structure-function relationship and its role in pathogenicity, scientists hope to identify potential drug targets that could disrupt bacterial survival, providing a crucial step towards combating multi-drug-resistant infections.
