Analytical Data
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Gene name
ROBO1
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简介
The ROBO1 protein is a receptor for SLIT1 and SLIT2 and critically mediates cellular responses during migration and axonal navigation. Interaction with FLRT3 promotes axonal attraction to NTN1-expressing cells. ROBO1 Protein, Rat (HEK293, His) is the recombinant rat-derived ROBO1 protein, expressed by HEK293 , with C-10*His labeled tag.
- Application
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Biological Activity
Measured in a cell proliferation assay using HUVEC cells. The ED50 for this effect is typically 48.92 ng/mL, corresponding to a specific activity is 2.04×104 units/mg. Measured in a cell proliferation assay using HUVEC cells. The ED50 for this effect is typically 48.92 ng/mL, corresponding to a specific activity is 2.04×104 units/mg.
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Alternative Names
DUTT1; DUTT1Drosophila) homolog 1; H-Robo-1; MGC131599; MGC133277; ROBO1; roundabout, axon guidance receptor, homolog 1 (Drosophila)
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Species
Rat
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Source
HEK293
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Tag
C-10*His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
O55005
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Expression Region
K19-I560
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AA Sequence
KKHLLLAQLIPDPEDLERGNDNGTPAPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYICVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESKVADVTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTFGWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTVTNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLTLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLESGVLQIRYAKLGDTGRYTCTASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLI
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Protein Length
Partial
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Molecular Weight
70-95 kDa.
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The study of ROBO1 (Roundabout Guidance Receptor 1) recombinant proteins has garnered significant attention in the fields of neuroscience and developmental biology, primarily due to its crucial role in axon guidance and neuron migration during the embryonic development of the nervous system. ROBO1 is a member of the ROBO family of receptors, which are involved in the regulation of neuronal connections and the establishment of appropriate pathways for growing axons. Disruptions in ROBO1 function have been implicated in various neurological disorders and abnormalities, including autism spectrum disorders and congenital heart defects, highlighting its importance in both neural and vascular development. Researchers seek to produce recombinant ROBO1 proteins to better understand its structure, function, and interactions with ligands such as Slit proteins, which are known to influence cellular behavior. These studies typically employ techniques such as protein expression in heterologous systems, purification, and functional assays to elucidate the signaling mechanisms and biological roles of ROBO1. Furthermore, the development of ROBO1 recombinant proteins opens avenues for therapeutic interventions that could rectify the pathological effects of ROBO1 mutations. Overall, the investigation of ROBO1 recombinant proteins not only enhances our comprehension of fundamental biological processes but also offers potential applications in clinical research and the development of targeted treatments for related disorders.
