Analytical Data
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Gene name
APPBP1-UBA3/NEDD8 E1
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简介
The APPBP1-UBA3/NEDD8 E1 protein is the regulatory subunit in the UBA3-NAE1 E1 enzyme complex and activates NEDD8 by adenylating its C-terminal glycine with ATP. This forms the NEDD8-UBA3 thioester, which is transferred to the catalytic cysteine of UBE2M. APPBP1-UBA3/NEDD8 E1 Protein, Human is a recombinant protein dimer complex containing human-derived APPBP1-UBA3/NEDD8 E1 protein, expressed by E. coli , with tag free. APPBP1-UBA3/NEDD8 E1 Protein, Human, has molecular weight of 60 kDa (APP-BP1) 50 kDa (UBA3).
- Application
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Biological Activity
Recombinant Human APPBP1/UBA3 Complex (NEDD8 E1) Protein is a member of the NEDD8-activating (E1) enzyme family that is required for the first step of the enzymatic cascade. Subsequently, NEDD8 is coupled to NEDD8-conjugating (E2) enzyme by forming a thioester bond. In this experiment, we used Recombinant Human APPBP1/UBA3 Complex (NEDD8 E1) Protein concentration of 80 nM, reaction conditions will need to be optimized for each specific application. Recombinant Human APPBP1/UBA3 Complex (NEDD8 E1) Protein is a member of the NEDD8-activating (E1) enzyme family that is required for the first step of the enzymatic cascade. Subsequently, NEDD8 is coupled to NEDD8-conjugating (E2) enzyme by forming a thioester bond. In this experiment, we used Recombinant Human APPBP1/UBA3 Complex (NEDD8 E1) Protein concentration of 80 nM, reaction conditions will need to be optimized for each specific application.
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Alternative Names
NAE1; HPP1; Ula-1; NEDD8-Activating Enzyme E1 Regulatory Subunit 1; Amyloid protein-binding protein 1; Proto-oncogene protein 1
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Species
Human
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Source
E. coli
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Tag
Tag Free
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
Q13564-1 (M1-L534)&Q8TBC4-1
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Expression Region
Q13564-1 (M1-L534)&Q8TBC4-1 (M22-S463)
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Molecular Weight
60 kDa (APP-BP1) 50 kDa (UBA3)
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The APPBP1-UBA3/NEDD8 E1 enzyme complex plays a crucial role in the neddylation pathway, a post-translational modification process that conjugates the small ubiquitin-like protein NEDD8 to target proteins, thereby regulating various cellular functions such as cell cycle progression, signal transduction, and protein stability. Dysregulation of neddylation has been implicated in several diseases, including cancer, neurodegenerative disorders, and viral infections. Research has increasingly focused on the APPBP1-UBA3 enzyme, which serves as the E1 activating enzyme in this pathway, as it is the first and essential step for NEDD8 activation and transfer. Understanding the structure and function of APPBP1-UBA3/NEDD8 is vital for elucidating its role in cellular processes and its potential as a therapeutic target. Recent studies using advanced techniques like X-ray crystallography and cryo-electron microscopy have provided insights into the molecular mechanisms of NEDD8 activation and its interaction with substrates. Furthermore, inhibitors targeting the APPBP1-UBA3 complex are being explored for their potential to disrupt aberrant neddylation in disease contexts, highlighting the importance of this enzyme in both fundamental biology and clinical applications.
