Analytical Data
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Gene name
METAP1/Methionine aminopeptidase 1
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简介
METAP1 (or methionine aminopeptidase 1) plays a crucial role in protein synthesis by co-translationally removing N-terminal methionine from nascent proteins.This enzymatic activity is particularly relevant when the second residue in the primary sequence is small and uncharged (including residues such as Ala, Cys, Gly, Pro, Ser, Thr or Val).METAP1/Methionine aminopeptidase 1 Protein, Human is the recombinant human-derived METAP1/Methionine aminopeptidase 1 protein, expressed by E.coli , with tag free.
- Application
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Alternative Names
rHuMethionine aminopeptidase 1/METAP1; Methionine aminopeptidase 1; MAP 1; MetAP 1; Peptidase M 1; METAP1
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Species
Human
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Source
E. coli
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Tag
Tag Free
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P53582
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Expression Region
M1-F386
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AA Sequence
MAAVETRVCETDGCSSEAKLQCPTCIKLGIQGSYFCSQECFKGSWATHKLLHKKAKDEKAKREVSSWTVEGDINTDPWAGYRYTGKLRPHYPLMPTRPVPSYIQRPDYADHPLGMSESEQALKGTSQIKLLSSEDIEGMRLVCRLAREVLDVAAGMIKPGVTTEEIDHAVHLACIARNCYPSPLNYYNFPKSCCTSVNEVICHGIPDRRPLQEGDIVNVDITLYRNGYHGDLNETFFVGEVDDGARKLVQTTYECLMQAIDAVKPGVRYRELGNIIQKHAQANGFSVVRSYCGHGIHKLFHTAPNVPHYAKNKAVGVMKSGHVFTIEPMICEGGWQDETWPDGWTAVTRDGKRSAQFEHTLLVTDTGCEILTRRLDSARPHFMSQF
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Protein Length
Full Length
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Molecular Weight
38-50 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Methionine aminopeptidase 1 (METAP1) is an enzyme that plays a crucial role in protein maturation by catalyzing the removal of the N-terminal methionine from nascent proteins, which is essential for the proper functioning of many cellular processes. This enzyme is involved in various biological functions, including protein synthesis, regulation of cell signaling pathways, and maintenance of protein stability. Abnormalities in METAP1 activity have been linked to several diseases, including cancer and neurodegenerative disorders, making it a potential target for therapeutic interventions. Research on recombinant METAP1 has garnered significant interest due to its implications in understanding the mechanisms of protein processing and its role in disease pathology. Scientists express and purify METAP1 to study its enzymatic properties, interactions with substrates, and potential inhibitors. Through structural and functional analysis, insight can be gained into the enzyme's mechanism and its involvement in various biological contexts. The exploration of METAP1 not only enhances our understanding of fundamental biological processes but also opens avenues for developing novel therapeutic strategies to address diseases associated with dysregulated protein metabolism.
