Analytical Data
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基因名
TPK1
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简介
Thiamin pyrophosphokinase 1 (TPK1) functions as a homodimer and catalyzes the conversion of thiamine (Vitamin B1) to thiamine pyrophosphate (TDP), a cofactor and a transient intermediate carrier of the aldehyde group for some enzymes of the glycolytic and energy production pathways. TPK1 can also catalyze the phosphorylation of pyrithiamine to pyrithiamine pyrophosphate. TPK1 Protein, Human (HEK293, His) is the recombinant human-derived TPK1 protein, expressed by HEK293 , with C-6*His labeled tag.
- Application
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别名
Thiamin pyrophosphokinase 1; hTPK1; Placental protein 20; PP20; Thiamine pyrophosphokinase 1; TPK1
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种属
Human
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表达系统
HEK293
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标签
C-6*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
AAH68460.1
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表达区间
M1-S243
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氨基酸序列
MEHAFTPLEPLLSTGNLKYCLVILNQPLDNYFRHLWNKALLRACADGGANRLYDITEGERESFLPEFINGDFDSIRPEVREYYATKGCELISTPDQDHTDFTKCLKMLQKKIEEKDLKVDVIVTLGGLAGRFDQIMASVNTLFQATHITPFPIIIIQEESLIYLLQPGKHRLHVDTGMEGDWCGLIPVGQPCSQVTTTGLKWNLTNDVLAFGTLVSTSNTYDGSGVVTVETDHPLLWTMAIKS
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蛋白长度
Full Length
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分子量
30 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
TPK1, or the Tetratricopeptide Repeat Protein 1, has garnered significant attention in recent years due to its potential roles in various biological processes and diseases. Understanding its function and mechanisms is crucial, given its involvement in protein-protein interactions and cellular signaling pathways. Research indicates that TPK1 may play a key role in cellular responses to stress and contribute to the regulation of mitochondrial dynamics, which further emphasizes its importance in maintaining cellular homeostasis. Additionally, aberrations in TPK1 expression and function have been linked to various pathological conditions, including neurodegenerative diseases and cancer, underscoring its potential as a biomarker or therapeutic target. The recombinant expression of TPK1 enables researchers to study its structure and function in detail, offering insights into its biological significance and providing a foundation for the development of novel therapeutic strategies. Understanding TPK1 through recombinant protein studies can also facilitate the discovery of its interacting partners, thus shedding light on the complex networks of cellular function it participates in. Given the multifaceted roles of TPK1, further investigation into its properties and interactions holds promising implications for both fundamental biology and clinical applications. Such research endeavors are essential for elucidating the underlying mechanisms of diseases associated with TPK1 and ultimately advancing our knowledge in the field of molecular biology and therapeutic interventions.
