Analytical Data
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基因名
TIM-14
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简介
The TIM-14 protein is an important component of the PAM complex and is required for the transport of transit peptide-containing proteins from the inner membrane to the mitochondrial matrix using ATP. TIM-14 Protein, S.cerevisiae is the recombinant TIM-14 protein, expressed by E. coli , with tag free.
- Application
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别名
Mitochondrial import inner membrane translocase subunit TIM14; Presequencetranslocated associated motor subunit PAM18; PAM18; TIM14
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种属
Others
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表达系统
E. coli
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标签
Tag Free
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q07914
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表达区间
F99-K168
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蛋白长度
Partial
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分子量
9 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
TIM-14, also known as Timm 14, is a critical component of the mitochondrial protein import machinery, playing a pivotal role in the translocation of proteins into mitochondria. Research into TIM-14 has gained significant interest due to its involvement in mitochondrial function and its potential implications in various diseases, including neurodegenerative disorders. Mitochondria are essential organelles responsible for energy production and metabolic regulation, and any dysfunction in their protein import pathways can lead to cellular stress and apoptosis. TIM-14 acts as a co-chaperone that assists the translocase of the inner mitochondrial membrane (TIM) complex, facilitating the import of precursor proteins that are essential for mitochondrial biogenesis and maintenance. Understanding the molecular mechanisms of TIM-14 function may reveal insights into the pathology of mitochondrial diseases, improve therapeutic strategies, and identify novel drug targets. Ongoing studies are focusing on the structural biology of TIM-14, its interaction with other chaperones, and the broader implications of its activity on mitochondrial dynamics and cellular health, highlighting its relevance in both fundamental biology and clinical research.
