Analytical Data
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基因名
Wnt3a Surrogate
- Application
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生物活性
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 3.2-9.4 ng/mL. Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells with an ED50 of 5.2 ng/mL. The ED50 of human Wnt Surrogate from MCE's each Lot is lower than of Competitor P.
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别名
Wnt-3a; Wnt 3a; WNT3A protein; Wnt3a
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种属
Human
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表达系统
HEK293
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标签
C-hFc
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纯度
Greater than 90% as determined by SDS-PAGE.
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分子量
60-75 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Wnt3a surrogate recombinant protein research stems from the critical role of the Wnt signaling pathway in various biological processes, including embryonic development, cell proliferation, and differentiation. Wnt3a, a member of the Wnt protein family, is pivotal in mediating this signaling pathway by binding to Frizzled receptors and activating downstream signaling cascades. Dysregulation of Wnt3a has been implicated in several diseases, including cancer and degenerative disorders, making it a significant target for therapeutic strategies. However, the complexity of Wnt signaling and the challenges associated with studying membrane-bound proteins have necessitated the development of surrogate recombinant proteins that can mimic the activity of native Wnt3a. Researchers have focused on engineering these surrogates to enhance their stability, solubility, and functionality, allowing for more straightforward studies of Wnt signaling dynamics and potential drug development. Understanding the mechanisms of Wnt3a surrogate proteins not only aids in unraveling the complexities of Wnt signaling but also holds promise for innovative treatments in regenerative medicine and oncology.
