Recombinant Human Wnt3a Surrogate Protein (HEK293)，hFc

Wnt3a Surrogate

SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 3.2-9.4 ng/mL. Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells with an ED50 of 5.2 ng/mL. The ED50 of human Wnt Surrogate from MCE's each Lot is lower than of Competitor P.

Wnt-3a; Wnt 3a; WNT3A protein; Wnt3a

Human

HEK293

C-hFc

Greater than 90% as determined by SDS-PAGE.

60-75 kDa

< 1.0 EU per μg protein as determined by the LAL method.

Freeze-dried powder

PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.