Analytical Data
-
Gene name
PNPLA2
- Application
-
Alternative Names
Adipose triglyceride lipase Calcium-independent phospholipase A2 Desnutrin IPLA2-zeta Pigment epithelium-derived factor TTS2.2 Transport-secretion protein 2 Short name:TTS2
-
Species
Human
-
Source
E. coli
-
Tag
N- His-SUMO
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
Q96AD5
-
Expression Region
1-504aa
-
Molecular Weight
71.3 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PNPLA2, also known as adipose triglyceride lipase (ATGL), plays a crucial role in lipid metabolism by catalyzing the hydrolysis of triacylglycerols to generate free fatty acids and glycerol. This enzyme is predominantly expressed in adipose tissue and is vital for the mobilization of stored fats, particularly during periods of energy demand, such as fasting or vigorous exercise. Mutations in the PNPLA2 gene can lead to various metabolic disorders, including congenital lipodystrophy and obesity, thereby underscoring the enzyme’s importance in maintaining energy homeostasis. The study of recombinant PNPLA2 proteins offers valuable insights into the enzyme's structure-function relationships, regulatory mechanisms, and interaction with other metabolic pathways. Researchers are particularly interested in elucidating the precise catalytic mechanisms of PNPLA2, as well as its role in adipocyte differentiation and lipolysis. By producing and characterizing recombinant PNPLA2, scientists aim to develop potential therapeutic strategies for metabolic diseases related to lipids, enhancing our understanding of lipid metabolism and its implications for cardiovascular health and metabolic syndrome.
