Analytical Data
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基因名
PNPLA2
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简介
The PNPLA2 protein catalyzes the hydrolysis of triglycerides, preferably long-chain fatty acid esters, in lipid droplets. PNPLA2 Protein, Human (His-SUMO) is the recombinant human-derived PNPLA2 protein, expressed by E. coli , with N-SUMO, N-6*His labeled tag.
- Application
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种属
Human
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表达系统
E. coli
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标签
N-SUMO;N-6*His
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
Q96AD5-1
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表达区间
M1-L504
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氨基酸序列
MFPREKTWNISFAGCGFLGVYYVGVASCLREHAPFLVANATHIYGASAGALTATALVTGVCLGEAGAKFIEVSKEARKRFLGPLHPSFNLVKIIRSFLLKVLPADSHEHASGRLGISLTRVSDGENVIISHFNSKDELIQANVCSGFIPVYCGLIPPSLQGVRYVDGGISDNLPLYELKNTITVSPFSGESDICPQDSSTNIHELRVTNTSIQFNLRNLYRLSKALFPPEPLVLREMCKQGYRDGLRFLQRNGLLNRPNPLLALPPARPHGPEDKDQAVESAQAEDYSQLPGEDHILEHLPARLNEALLEACVEPTDLLTTLSNMLPVRLATAMMVPYTLPLESALSFTIRLLEWLPDVPEDIRWMKEQTGSICQYLVMRAKRKLGRHLPSRLPEQVELRRVQSLPSVPLSCAAYREALPGWMRNNLSLGDALAKWEECQRQLLLGLFCTNVAFPPEALRMRAPADPAPAPADPASPQHQLAGPAPLLSTPAPEARPVIGALGL
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蛋白长度
Full Length of Isoform-1
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分子量
71.3-73 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PNPLA2, also known as adipose triglyceride lipase (ATGL), plays a crucial role in lipid metabolism by catalyzing the hydrolysis of triacylglycerols to generate free fatty acids and glycerol. This enzyme is predominantly expressed in adipose tissue and is vital for the mobilization of stored fats, particularly during periods of energy demand, such as fasting or vigorous exercise. Mutations in the PNPLA2 gene can lead to various metabolic disorders, including congenital lipodystrophy and obesity, thereby underscoring the enzyme’s importance in maintaining energy homeostasis. The study of recombinant PNPLA2 proteins offers valuable insights into the enzyme's structure-function relationships, regulatory mechanisms, and interaction with other metabolic pathways. Researchers are particularly interested in elucidating the precise catalytic mechanisms of PNPLA2, as well as its role in adipocyte differentiation and lipolysis. By producing and characterizing recombinant PNPLA2, scientists aim to develop potential therapeutic strategies for metabolic diseases related to lipids, enhancing our understanding of lipid metabolism and its implications for cardiovascular health and metabolic syndrome.
