Analytical Data
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Gene name
EIF1AX
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简介
The EIF1AX protein is an important member of the 43S preinitiation complex (43S PIC) and is responsible for coordinating mRNA cap-proximal binding, scanning the 5'-untranslated region, and pinpointing the start codon. EIF1AX Protein, Human (His) is the recombinant human-derived EIF1AX protein, expressed by E. coli , with N-6*His labeled tag.
- Application
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Alternative Names
rHuEukaryotic translation initiation factor 1A, X-chromosomal/EIF1AX, His; Eukaryotic Translation Initiation Factor 1A X-Chromosomal; eIF-1A X Isoform; Eukaryotic Translation Initiation Factor 4C; eIF-4C; EIF1AX; EIF1A; EIF4C
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Species
Human
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Source
E. coli
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Tag
N-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P47813
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Expression Region
M1-I144
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Protein Length
Full Length
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Molecular Weight
22 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
EIF1AX (Eukaryotic Translation Initiation Factor 1 A) is a pivotal protein involved in the initiation of protein synthesis, playing a crucial role in the recruitment of the ribosome to mRNA. Recent studies have highlighted the importance of EIF1AX in various biological processes, including cell growth and proliferation, as well as its involvement in the regulation of gene expression. Dysregulation or mutations in the EIF1AX gene have been linked to several cancers, suggesting that it may act as an oncogene. Research has increasingly focused on the structural and functional analysis of EIF1AX, utilizing recombinant protein techniques to produce and characterize the protein for in-depth studies. By generating recombinant EIF1AX, researchers aim to elucidate its precise role in translation initiation and its interactions with other initiation factors and mRNA. Understanding the mechanistic pathways involving EIF1AX is critical for developing targeted therapies, particularly in cancer treatment, where its functional modulation could provide novel avenues for intervention. Given its significance, the exploration of EIF1AX as a therapeutic target is gaining momentum, driving forward the knowledge of protein synthesis regulation and its implications in disease contexts.
