Analytical Data
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Gene name
dUTPase/DUT
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简介
Deoxyuridine 5'-triphosphate nucleotidohydrolase (DUT) is an essential enzyme of nucleotide metabolism, as DUT hydrolyzes dUTP to dUMP and pyrophosphate, preventing uracil misincorporation into DNA efficiently and provides dUMP for thymidylate biosynthesis. DUT also prevents dimerization of PPAR and retinoid X receptor and is essential for embryonic development as well. dUTPase/DUT Protein, Human is the recombinant human-derived dUTPase/DUT protein, expressed by E. coli , with tag free.
- Application
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Alternative Names
rHuDeoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial/DUT; Deoxyuridine 5'-Triphosphate Nucleotidohydrolase Mitochondrial; dUTPase; dUTP Pyrophosphatase; DUT
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Species
Human
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Source
E. coli
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Tag
Tag Free
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P33316-2
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Expression Region
M1-N164
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Protein Length
Full Length of Isoform-2
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Molecular Weight
18.0 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
dUTPase (deoxyuridine triphosphate nucleotidohydrolase), an essential enzyme, plays a critical role in maintaining DNA integrity by preventing the incorporation of uracil into DNA during replication. It catalyzes the hydrolysis of dUTP to dUMP and pyrophosphate, thus regulating the intracellular levels of dUTP and fostering a balanced nucleotide pool essential for accurate DNA synthesis. Abnormal dUTPase activity has been linked to various diseases, including cancer, highlighting its potential as a therapeutic target. Moreover, understanding the structure and function of dUTPase is instrumental in deciphering its mechanisms and interactions within cellular pathways. Research on recombinant dUTPase/DUT proteins has gained momentum, utilizing techniques such as protein expression in bacterial systems, purification methods, and functional assays to elucidate its biochemical properties and interaction with other cellular components. Such studies not only advance our comprehension of nucleotide metabolism but also pave the way for the development of novel inhibitors that might serve as lead compounds in drug design. Integrating structural biology approaches, including X-ray crystallography and cryo-electron microscopy, complements these efforts, offering insights into the enzyme's active site and substrate binding. As a result, dUTPase continues to be a focal point in molecular biology, pharmacology, and biotechnology research, with implications spanning from basic science to clinical applications.
