Analytical Data
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基因名
Protease 7/OmpT
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简介
Protease 7/OmpT protein is a multifunctional enzyme that can cleave a variety of substrates, such as T7 RNA polymerase, ferric enterobactin receptor protein (FEP), and the antimicrobial peptide protamine. Its unique specificity for paired basic residues suggests a preference for specific amino acid configurations. Protease 7/OmpT Protein, E.coli (His) is the recombinant E. coli-derived Protease 7/OmpT protein, expressed by E. coli , with N-6*His labeled tag.
- Application
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别名
ompT; b0565; JW0554; Protease 7; EC 3.4.23.49; Omptin; Outer membrane protein 3B; Protease A; Protease VII
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种属
E.coli
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表达系统
E. coli
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标签
N-6*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P09169
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表达区间
S21-F317
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氨基酸序列
STETLSFTPDNINADISLGTLSGKTKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMVDQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYSFTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTFKYSGWVESSDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRVTNKKGNTSLYDHNNNTSDYSKNGAGIENYNFITTAGLKYTF
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蛋白长度
Full Length of Mature Protein
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分子量
37.5 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Protease 7, also known as OmpT (Outer Membrane Protein T), is a member of the type 1 serine protease family and is primarily found in the outer membrane of certain Gram-negative bacteria, including Escherichia coli. This protein plays a crucial role in the bacterial life cycle by participating in the degradation of peptide bonds, which is essential for nutrient acquisition and regulation of protein turnover. The ability of OmpT to cleave various substrates has garnered significant interest in the fields of microbiology and biotechnology, as it can be utilized in the development of therapeutic agents and diagnostic tools. Furthermore, recombinant forms of OmpT have been explored for their potential to enhance the delivery and efficacy of various biomolecules and vaccines, owing to their unique properties. Research into the structural and functional aspects of Protease 7/OmpT has provided valuable insights into its enzymatic mechanisms, substrate specificity, and potential applications in biocatalysis. Understanding the regulation and expression of this protease is also critical, as it could offer novel strategies for combating antibiotic resistance, which is a growing global concern. Given the increasing relevance of biotechnological applications, studies investigating the re-engineering of OmpT for improved performance in diverse industrial processes are actively being pursued. Overall, Protease 7/OmpT represents a compelling target for ongoing research, with implications reaching far beyond its native bacterial context, potentially revolutionizing approaches in drug development and molecular biology.
