Analytical Data
-
Gene name
PEPD
-
简介
PEPD Protein, a member of the peptidase family, is a dipeptidase which hydrolyze dipeptides with proline or hydroxyproline at the carboxy terminus. It plays an important role in collagen metabolism because the high level of iminoacids in collagen. PEPD suppression of p53 is essential for cell survival and tumor growth. PEPD Protein, Human is the recombinant human-derived PEPD protein, expressed by E. coli , with tag free.
- Application
-
Alternative Names
Xaa-Pro dipeptidase; Imidodipeptidase; PeptidaseD; Prolinedipeptidase; PRD; PEPD.
-
Species
Human
-
Source
E. coli
-
Tag
Tag Free
-
Purity
Greater than 95% as determined by SDS-PAGE.
-
Uniprot
AAH28295.1
-
Expression Region
A2-K493
-
AA Sequence
AAATGPSFWLGNETLKVPLALFALNRQRLCERLRKNPAVQAGSIVVLQGGEETQRYCTDTGVLFRQESFFHWAFGVTEPGCYGVIDVDTGKSTLFVPRLPASHATWMGKIHSKEHFKEKYAVDDVQYVDEIASVLTSQKPSVLLTLRGVNTDSGSVCREASFDGISKFEVNNTILHPEIVECRVFKTDMELEVLRYTNKISSEAHREVMKAVKVGMKEYELESLFEHYCYSRGGMRHSSYTCICGSGENSAVLHYGHAGAPNDRTIQNGDMCLFDMGGEYYCFASDITCSFPANGKFTADQKAVYEAVLRSSRAVMGAMKPGVWWPDMHRLADRIHLEELAHMGILSGSVDAMVQAHLGAVFMPHGLGHFLGIDVHDVGGYPEGVERIDEPGLRSLRTARHLQPGMVLTVEPGIYFIDHLLDEALADPARASFFNREVLQRFRGFGGVRIEEDVVVTDSGIELLTCVPRTVEEIEACMAGCDKAFTPFSGPK
-
Protein Length
Full Length of Mature Protein
-
Molecular Weight
54-60 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PEPD (peptidase D) is an essential enzyme that plays a critical role in the degradation of oligopeptides and the regulation of peptide signaling in various biological processes. Research into PEPD has gained momentum due to its involvement in several physiological functions, including immune response, cellular signaling, and metabolism. Abnormalities in PEPD expression or function have been linked to various diseases, including neurodegenerative disorders and cancer, making it a potential target for therapeutic interventions. The recombination of PEPD proteins has emerged as a significant focus, allowing scientists to study its structure-function relationships more precisely. By utilizing recombinant DNA technology, researchers can produce high yields of PEPD for functional assays and structural studies, enabling a deeper understanding of its enzymatic mechanisms and interactions with different substrates. This research is crucial for developing novel strategies for disease treatment and improving our understanding of peptide metabolism and its implications in health and disease.
