Cat: IPD-X20415

Recombinant Rat CD40 Protein(HEK293), His

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Analytical Data

  • Gene name

    CD40

  • 简介

    CD40, also known as TNFR5, is a protein that exhibits defects in conserved residues necessary for feature annotation propagation. Specific residues missing in CD40 prevent the propagation of certain functional features associated with this protein. CD40 Protein, Rat (HEK293, His) is the recombinant rat-derived CD40 protein, expressed by HEK293 , with C-His labeled tag.

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Biological Activity

    Measured by its binding ability in a functional ELISA. When Recombinant Rat CD40/TNFRSF5 is immobilized at 5 µg/mL (100 µL/well) can bind Recombinant Human CD40 Ligand/TNFSF5. The ED50 for this effect is 668.1 ng/mL.

  • Alternative Names

    Tumor Necrosis Factor Receptor Superfamily member 5; Bp50; CD40L Receptor; CDw40; TNFRSF5

  • Species

    Rat

  • Source

    HEK293

  • Tag

    C-His

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    A6JXD6

  • Expression Region

    L20-R193

  • Protein Length

    Partial

  • Molecular Weight

    28 kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

Quality inspection process

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Protein Description

CD40 is a co-stimulatory protein found on the surface of various immune cells, including B cells, dendritic cells, and macrophages. It plays a crucial role in regulating immune responses and facilitating T cell activation, making it a significant target for therapeutic intervention in various diseases. The study of recombinant CD40 proteins has gained momentum in immunology due to their potential in vaccine development, cancer therapy, and autoimmune disease treatment. Recombinant CD40 and its ligands can enhance the activation and proliferation of immune cells, improving their ability to respond to pathogens or tumors. By examining the structure and function of CD40, researchers aim to understand its mechanisms and optimize its use in clinical applications. Additionally, exploring CD40's interactions with other immune pathways can help identify new strategies for modulating immune responses. Given its pivotal role in promoting immunity and maintaining homeostasis, CD40 remains a focal point of research aimed at harnessing its properties for therapeutic benefits. The advancement of recombinant DNA technology has facilitated the production of this protein, allowing for detailed study and application in various biomedical fields, thus highlighting the importance of ongoing research into CD40 recombinant proteins.

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