Recombinant Human CD28 Protein(HEK293)

CD28

The CD28 protein is critical for T cell activation, enhancing proliferation, cytokine production, and survival. When linked to TCR/CD3 and CD40L, CD28 promotes the production of IL4 and IL10, thereby regulating immune responses. CD28 Protein, Human/Cynomolgus/Rhesus Macaque (HEK293) is the recombinant human-derived CD28 protein, expressed by HEK293 , with tag free.

SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

Measured by its binding ability in a functional ELISA. Immobilized Human CD28 at 1 μg/mL (100 μL/well) can bind Biotinylated Human B7-1 protein. The ED50 for this effect is 521.3 ng/mL. Measured by its binding ability in a functional ELISA. Immobilized Human CD28 at 1 μg/mL (100 μL/well) can bind Biotinylated Human B7-1 protein. The ED50 for this effect is 521.3 ng/mL。

CD28; T-cell-specific surface glycoprotein CD28; Tp44

Human

HEK293

Tag Free

Greater than 90% as determined by SDS-PAGE.

P10747-1/Q0PDN3

N19-P152

Extracellular Domain

34-44 kDa

< 1.0 EU per μg protein as determined by the LAL method.

Freeze-dried powder

PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.