Analytical Data
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Gene name
CD3 zeta/CD247
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简介
CD3 zeta/CD247 is a component of the TCR-CD3 complex and can transmit APC-induced TCR signals to initiate immune responses. CD3D, CD3E, CD3G, and CD3Z contain ITAMs that are phosphorylated by LCK and FYN upon TCR engagement, providing docking sites for ZAP70. CD3 zeta/CD247 Protein, Human (GST) is the recombinant human-derived CD3 zeta/CD247 protein, expressed by E. coli , with N-GST labeled tag.
- Application
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Biological Activity
Measured by its ability to inhibit the cell growth of Jurkat cells. The ED50 for this effect is 198.5 ng/mL, corresponding to a specific activity is 5.038×103 units/mg. Measured by its ability to inhibit the cell growth of Jurkat cells. The ED50 for this effect is 198.5 ng/ml, corresponding to a specific activity is 5.038×103 units/mg.
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Alternative Names
T-cell surface glycoprotein CD3 zeta chain; CD247; CD3Z; T3Z; TCRZ
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P20963-1
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Expression Region
R52-R164
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Protein Length
Cytoplasmic Domain
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Molecular Weight
16 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CD3 zeta (CD247) is a critical component of the T-cell receptor (TCR) complex, playing a vital role in T-cell activation and signaling. The CD3 complex, which consists of CD3 gamma, delta, epsilon, and zeta chains, is essential for transmitting activation signals upon antigen binding. Dysregulation or mutations in CD247 can lead to various immunological disorders, including immunodeficiencies, autoimmunity, and malignancies. Research on recombinant CD3 zeta proteins aims to enhance our understanding of T-cell functionality and improve therapeutic interventions. For instance, recombinant CD3 zeta can be utilized in the development of biopharmaceuticals that modulate immune responses, such as in cancer immunotherapy where reactivation of T cells is essential for targeting tumor cells. Additionally, studying CD3 zeta in a recombinant form allows researchers to investigate its specific interactions with other components of the immune system, paving the way for innovative treatment strategies for immune-related diseases. By leveraging recombinant technologies, scientists can optimize the design and function of CD3 zeta, potentially leading to advancements in personalized medicine and the treatment of various immunological conditions. Overall, research on CD3 zeta/CD247 recombinant proteins is vital for elucidating T-cell biology and developing novel therapeutic approaches.
