Analytical Data
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基因名
IgE
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简介
IgE protein is an important immunoglobulin component that participates in the recognition and effector phases of humoral immunity. As a membrane-bound receptor on B lymphocytes, IgE triggers clonal expansion upon antigen binding, leading to plasma cell differentiation. IgE Protein, Human (HEK293, His) is the recombinant human-derived IgE protein, expressed by HEK293 , with C-His labeled tag.
- Application
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生物活性
1. Measured by its binding ability in a functional ELISA. When Recombinant Human IgE Protein is immobilized at 2 µg/mL (100 µL/well) can bind Biotinylated Human CD23 Protein. The ED50 for this effect is 0.5-3.5 μg/mL. 2. Loaded Ligelizumab (HY-P99300) on AHC2 biosensor, can bind IgE Protein, Human (HEK293, His) with an affinity constant of 1.089E-12 M as determined in BLI assay. Measured by its binding ability in a functional ELISA. When Recombinant Human IgE Protein is immobilized at 2 µg/mL (100 µL/well) can bind Biotinylated Human CD23 Protein. The ED50 for this effect is 0.7468 μg/mL. Loaded Ligelizumab (HY-P99300) on AHC2 biosensor, can bind IgE Protein, Human (HEK293, His) with an affinity constant of 1.089E-12 M as determined in BLI assay.
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别名
Immunoglobulin heavy constant epsilon; IGHE; IgE
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种属
Human
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表达系统
HEK293
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标签
C-His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
AAB59395
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表达区间
C208-K427
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氨基酸序列
CADSNPRGVSAYLSRPSPFDLFIRKSPTITCLVVDLAPSKGTVNLTWSRASGKPVNHSTRKEEKQRNGTLTVTSTLPVGTRDWIEGETYQCRVTHPHLPRALMRSTTKTSGPRAAPEVYAFATPEWPGSRDKRTLACLIQNFMPEDISVQWLHNEVQLPDARHSTTQPRKTKGSGFFVFSRLEVTRAEWEQKDEFICRAVHEAASPSQTVQRAVSVNPGK
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蛋白长度
Partial
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分子量
26-35 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Immunoglobulin E (IgE) is a key antibody involved in allergic reactions and responses to parasitic infections. The study of IgE and its recombinant forms has gained significant attention due to the increasing prevalence of allergic diseases globally, including asthma, eczema, and food allergies. Understanding the structure and function of IgE at a molecular level is crucial for developing targeted therapies and vaccines. Recombinant IgE proteins offer the potential for studying the allergenic properties of various antigens, elucidating the mechanisms of IgE-mediated signaling, and identifying novel therapeutic targets. By engineering specific IgE variants, researchers aim to investigate their binding affinities to allergens and assess their roles in triggering mast cell activation and subsequent inflammation. Furthermore, recombinant IgE can be utilized in the development of sensitive diagnostic tools for allergy detection and in the design of immunotherapies aimed at modulating IgE responses. This research is not only pivotal for advancing allergy treatments but also contributes to broader immunological insights and the potential for innovative approaches in managing immune-related disorders.
